Chinese Medical Sciences Journal ›› 2010, Vol. 25 ›› Issue (4): 237-242.doi: 10.1016/S1001-9294(11)60009-6

• Original Article • 上一篇    下一篇

Ellagic acid-induced hypercoagulable state in animals: a potentially useful animal hypercoagulable model for evaluation of anticoagulants

Na Liu, Jun-tian Liu*, and Qiang-zong Zhang   

  1. Department of Pharmacology, Xi’an Jiaotong University, Xi’an 710061, China
  • 收稿日期:2010-06-10 出版日期:2010-12-20 发布日期:2010-12-20
  • 通讯作者: *Correspondingauthor Tel: 86-29-82655188, E-mail: ljt@mail.xjtu. edu.cn E-mail:ljt@mail.xjtu. edu.cn

Ellagic acid-induced hypercoagulable state in animals: a potentially useful animal hypercoagulable model for evaluation of anticoagulants

Na Liu, Jun-tian Liu*, and Qiang-zong Zhang   

  1. Department of Pharmacology, Xi’an Jiaotong University, Xi’an 710061, China
  • Received:2010-06-10 Online:2010-12-20 Published:2010-12-20
  • Contact: Department of Pharmacology, Xi’an JiaotongUniversity School of Medicine, Xi’an 710061, China E-mail:ljt@mail.xjtu. edu.cn

摘要: Objective To establish and evaluate a hypercoagulable animal model for the assessment of anticoagulants. Methods Forty mice, thirty-two rats, and twenty-four rabbits were randomly and equally divided into control group (saline) and three ellagic acid (EA)-treated groups (low, middle, and high doses). In the mice, bleeding time (BT) was estimated with tail transaction, and clotting time (CT) with template method. Prothrombin time (PT) and the activated partial thromboplastin time (APTT) in rats and rabbits were measured by means of Quick’s one-stage assay and modified APTT assay respectively. In addition, thrombin activity was estimated in rats with PT assay using a hemagglutination analyzer. The circulating platelet aggregates were detected in rabbits through platelet counting and presented as the circulating platelet aggregate ratio (CPAR). Results EA shortened BT and CT in mice, PT and APTT in rats, and increased thrombin activity and CPAR, all in a dose-dependent manner. EA also brought reduction of PT and APTT in rabbits in dose- and time-dependent manners. Conclusion EA could induce hypercoagulable state through activating coagulation system and platelets in mice, rats, and rabbits.

关键词: ellagic acid, hypercoagulablestate;coagulation, platelet aggregation

Abstract: Objective To establish and evaluate a hypercoagulable animal model for the assessment of anticoagulants. Methods Forty mice, thirty-two rats, and twenty-four rabbits were randomly and equally divided into control group (saline) and three ellagic acid (EA)-treated groups (low, middle, and high doses). In the mice, bleeding time (BT) was estimated with tail transaction, and clotting time (CT) with template method. Prothrombin time (PT) and the activated partial thromboplastin time (APTT) in rats and rabbits were measured by means of Quick’s one-stage assay and modified APTT assay respectively. In addition, thrombin activity was estimated in rats with PT assay using a hemagglutination analyzer. The circulating platelet aggregates were detected in rabbits through platelet counting and presented as the circulating platelet aggregate ratio (CPAR). Results EA shortened BT and CT in mice, PT and APTT in rats, and increased thrombin activity and CPAR, all in a dose-dependent manner. EA also brought reduction of PT and APTT in rabbits in dose- and time-dependent manners. Conclusion EA could induce hypercoagulable state through activating coagulation system and platelets in mice, rats, and rabbits.

Key words: ellagic acid, hypercoagulablestate;coagulation, platelet aggregation

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