Chinese Medical Sciences Journal ›› 2014, Vol. 29 ›› Issue (2): 103-106.doi: 10.1016/S1001-9294(14)60036-5

• 论著 • 上一篇    下一篇

False Human Immunodeficiency Virus Test Results Associated with Rheumatoid Factors in Rheumatoid Arthritis

Yun-chun Li*, Fan Yang, Xiao-yun Ji, Zhong-jun Fang, Jun Liu, Yue Wang   

  1. Department of Laboratory Center, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai 200052, China
  • 收稿日期:2013-07-12 出版日期:2014-06-26 发布日期:2014-06-26

False Human Immunodeficiency Virus Test Results Associated with Rheumatoid Factors in Rheumatoid Arthritis

Yun-chun Li*, Fan Yang, Xiao-yun Ji, Zhong-jun Fang, Jun Liu, Yue Wang   

  1. Department of Laboratory Center, Shanghai Guanghua Hospital of Integrative Medicine, Shanghai 200052, China
  • Received:2013-07-12 Online:2014-06-26 Published:2014-06-26
  • Contact: Tel: 86-21-62805833-3202, E-mail: liyunchun06 @163.com

摘要: Objective To investigate if immunological factors associated with rheumatoid arthritis (RA) affect the result of human immunodeficiency virus (HIV) screening by electrochemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA).Methods 100 RA cases were enrolled from January 2012 to February 2013 into this study. HIV screening was conducted with ECLIA detecting both HIV-1 p24 antigen, HIV-1 and HIV-2 antibodies, with ELISA and colloidal gold method detecting HIV-1 and HIV-2 antibodies. The samples producing positive results were submitted to the Center for Disease Control for confirmation using Western blotting method. The antibody titers of rheumatoid factors (RF) including RF-IgG, RF-IgM, RF-IgA, and CCP-IgG were analyzed by ELISA.Results The HIV positive-rate determined by ECLIA was significantly higher than that by ELISA and colloidal gold method (P<0.01). The false-positive rate of HIV screening was associated with antibody titers of RF-IgG, RF-IgM, RF-IgA, and CCP-IgG in RA (P<0.01).Conclusions Immunological factors, including RF and anti-CCP antibody, may influence the screening of HIV by ECLIA, producing false-positive result.

关键词: human immunodeficiency virus, false positive, rheumatoid arthritis, antibody

Abstract: Objective To investigate if immunological factors associated with rheumatoid arthritis (RA) affect the result of human immunodeficiency virus (HIV) screening by electrochemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA).Methods 100 RA cases were enrolled from January 2012 to February 2013 into this study. HIV screening was conducted with ECLIA detecting both HIV-1 p24 antigen, HIV-1 and HIV-2 antibodies, with ELISA and colloidal gold method detecting HIV-1 and HIV-2 antibodies. The samples producing positive results were submitted to the Center for Disease Control for confirmation using Western blotting method. The antibody titers of rheumatoid factors (RF) including RF-IgG, RF-IgM, RF-IgA, and CCP-IgG were analyzed by ELISA.Results The HIV positive-rate determined by ECLIA was significantly higher than that by ELISA and colloidal gold method (P<0.01). The false-positive rate of HIV screening was associated with antibody titers of RF-IgG, RF-IgM, RF-IgA, and CCP-IgG in RA (P<0.01).Conclusions Immunological factors, including RF and anti-CCP antibody, may influence the screening of HIV by ECLIA, producing false-positive result.

Key words: human immunodeficiency virus, false positive, rheumatoid arthritis, antibody

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