Chinese Medical Sciences Journal ›› 2018, Vol. 33 ›› Issue (4): 252-259.doi: 10.24920/003477

• 论著 • 上一篇    下一篇

纵向测量大鼠非动脉炎性前部缺血性视神经病变模型中后部视乳头血流变化的研究

马瑾1,陈婷1,2,王一玮1,赵潺1,李东辉1,王萌1,干霖洋1,钟勇1,*   

  1. 1 中国医学科学院北京协和医学院北京协和医院眼科
    2 武汉大学人民医院眼科中心
  • 收稿日期:2018-05-07 接受日期:2018-09-03 出版日期:2018-12-30 发布日期:2018-12-10
  • 通讯作者: 钟勇

Longitudinal Measurement of Hemodynamic Changes within the Posterior Optic Nerve Head in Rodent Nonarteritic Anterior Ischemic Optic Neuropathy

Ma Jin1,Chen Ting1,2,Wang Yiwei1,Zhao Chan1,Li Donghui1,Wang Meng1,Gan Linyang1,Zhong Yong1,*   

  1. 1 Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China;
    2 Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, 430060 China
  • Received:2018-05-07 Accepted:2018-09-03 Published:2018-12-30 Online:2018-12-10
  • Contact: Zhong Yong

摘要:

目的 活体评估大鼠非动脉炎性前部缺血性视神经病变(Rat nonarteriticanterior ischemic optic neuropathy, rNAION)模型后部视乳头血流灌注的动态变化。 方法 采用SD 鼠孟加拉玫瑰红股静脉注射和氩绿激光法制作rNAION模型。通过眼底彩照和荧光素钠血管造影(Fundus fluorescein angiography, FFA)分别评估造模后视神经90天内形态变化和3小时内的血供变化,采用组织形态学来判断模型是否成功建立,采用激光多普勒血流仪(Laser doppler flowmetry, LDF)在造模后第3天、7天、14天、21天和40天测量后部视乳头血流动力学的动态变化。统计分析采用单因素方差分析, Student’s t检验以及Bonferroni校正法进行多重比较。 结果 在rNAION模型中观察到视乳头水肿、消退与视盘苍白密切相关。FFA显示早期视盘低荧光,晚期高荧光。组织学研究显示视乳头组织水肿、疏松,视网膜神经节细胞和神经纤维大量丢失,大量神经胶质增生。与正常大鼠相比,造模后大鼠各观察时间点血流动力学参数值均明显降低(F=175.06,P<0.0001),以在造模后14天以内下降最为显著(AllP<0.01)。 结论 rNAION模型病程中后部视乳头血流灌注持续降低,且在疾病发生14天前变化最为明显。该结果为探明rNAION模型中血流动态变化提供重要参考信息。

关键词: 非动脉炎性前部缺血性视神经病变, 大鼠模型, 后部视乳头, 血流变化, 活体

Abstract:

Objective To assess the in vivo dynamic blood flow features of posterior optic nerve head (ONH) in rat model of nonarteritic anterior ischemic optic neuropathy (rNAION). Methods rNAION was established with Rose Bengal and argon green laser in Sprague-Dawley rats. Fundus photography and fundus fluorescein angiography (FFA) were performed to assess the dynamic changes of optic disc in morphology in 90 days and in blood perfusion in 3 hours after the induction of disease. Histological examinations were performed to evaluate the success of modeling. The dynamic blood flow kinetics of posterior ONH in rNAION were measured by Laser Doppler Flowmetry (LDF) on the day 3, 7, 14, 21, and 40 after the disease induction. One-way ANOVA, Student’s t-test and Bonferroni adjustment were used for multiple comparisons of kinetic measurements of blood flow. Results Optic disc edema and subsequent resolution associated with the development of optic disc pallor were observed in rNAION. FFA showed that the optic disc was hypofluorescence in the early phase and hyperfluorescence in the late phase. Histological studies suggested edema and loosened tissues of ONH, loss of retinal ganglion cells (RGCs), optic nerve substance and gliosis. Compared to the naive rats, the blood flow kinetics of posterior ONH in rNAION significant reduced at each time point after modeling (F=175.06, P<0.0001). The reductions were specifically remarkable in 14 days after the disease induction (All P<0.01). Conclusions Continuous blood perfusion reduction was found in rNAION, with significant alteration in 14 days after disease induction. Our results provided important information for understanding the hemodynamic changes in rNAION.

Key words: nonarteritic anterior ischemic optic neuropathy, rat model, posterior optic nerve head, blood flow, in vivo

基金资助: 国家自然科学基金(81670854)

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