Chinese Medical Sciences Journal ›› 2019, Vol. 34 ›› Issue (4): 281-288.doi: 10.24920/003495

• 技术方法 • 上一篇    下一篇

氧化偶氮甲烷-硫酸葡聚糖硫酸钠诱导小鼠结直肠肿瘤方法的优化

梁玺,胡婧楠,何建明   

  1. 河北中医学院河北省中医院放射治疗科,石家庄市050011 中国
  • 收稿日期:2018-08-09 接受日期:2018-10-10 出版日期:2019-12-31 发布日期:2019-12-04
  • 通讯作者: 梁玺,胡婧楠,何建明

An Optimized Protocol of Azoxymethane-Dextran Sodium Sulfate Induced Colorectal Tumor Model in Mice

Liang Xi,Hu Jingnan,He Jianming   

  1. Department of Radiotherapy, Hebei Province Hospital of Chinese Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050011, China
  • Received:2018-08-09 Accepted:2018-10-10 Published:2019-12-31 Online:2019-12-04
  • Contact: Liang Xi,Hu Jingnan,He Jianming

摘要:

自氧化偶氮甲烷(AOM)-硫酸葡聚糖硫酸钠(DSS)诱导肿瘤用于研究炎症相关或散发性结直肠癌(CRC)以来,数种AOM、DSS处理方式被报导。本文优化了AOM-DSS诱导C57BL/6小鼠散发性结直肠癌模型的方法。利用100 μl消毒注射器按照需要剂量一次性腹腔注射适当浓度AOM,DSS处理7天并每天更换新鲜的DSS液体。我们共诱导超过100只小鼠,AOM处理后8至20周时,发现每只小鼠均被诱导发生结直肠肿瘤。肿瘤分布以结直肠远端居多,与人类结直肠癌相似。采用此方法建模的每只小鼠的成瘤数量较多、鼠间肿瘤数量差异较小。此AOM-DSS模型中的肿瘤病变涵盖了瘤前病变、腺瘤性病变、腺瘤以及腺癌。本改良的AOM-DSS结直肠癌建模方法具有简单、经济、成瘤率高等优点。

关键词: 动物模型, 结直肠癌, 炎症, 方法

Abstract:

Since azoxymethane (AOM)-dextran sodium sulfate (DSS) induced tumorigenesis was used to explore inflammation-associated carcinogenesis of sporadic colorectal cancer (CRC), different administration modes of AOM or DSS have been reported. In this article we optimized the protocol of the AOM-DSS modeling using C57BL/6 mice for study on sporadic CRC by intraperitoneal injecting AOM solution at a proper concentration with a 100 μl sterile syringe once, feeding with DSS solution for 7 days in a roll and change DSS solution every day. More than 100 C57BL/6 mice had been treated with the optimized protocol, and all mice were demonstrated suffering from colorectal tumors when sacrificed in 8 to 20 weeks after AOM injection. These tumors mainly occurred in distal segment of colorectum with an increase in tumor density, which was similar to CRC in human beings. Tumor per mouse was high, and variation of tumor number per mouse was low. The histology of tumor developed through the defined stage ranged from precursor lesions, adenomatous lesions, adenomas to adenocarcinomas. The modified protocol of AOM-DSS model is easy, cheap, with high tumor formation rate of colorectal tumors.

Key words: animal model, colorectal cancer, inflammation, protocol

基金资助: 河北省卫生与计划生育委员会科研基金项目(20180688)

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