Chinese Medical Sciences Journal ›› 2020, Vol. 35 ›› Issue (1): 85-91.doi: 10.24920/003532

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伴血脂异常2型糖尿病患者亚甲基四氢叶酸还原酶基因多态性与血浆同型半胱氨酸水平的相关性

尹影1,李蕊2,李晓黎2,吴坤荣2,李玲2,徐曰东4,廖琳4,杨蕊3,李妍3,*()   

  1. 1山东第一医科大学(山东省医学科学院)药学院,山东,泰安,271000
    2山东大学药学院,山东,济南,250014
    3山东省千佛山医院药剂科,山东,济南,250014
    4内分泌科,山东,济南,250014
  • 收稿日期:2019-05-12 出版日期:2020-03-31 发布日期:2020-04-16
  • 通讯作者: 李妍 E-mail:li_xyan@126.com

Association Between Homocysteine Level and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Type 2 Diabetes Accompanied by Dyslipidemia

Yin Ying1,Li Rui2,Li Xiaoli2,Wu Kunrong2,Li Ling2,Xu Yuedong4,Liao Lin4,Yang Rui3,Li Yan3,*()   

  1. 1School of Pharmaceutical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Tai’an, Shandong 271000, China
    2School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
    3Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China
    4Department of Endocrinology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China
  • Received:2019-05-12 Online:2020-03-31 Published:2020-04-16
  • Contact: Li Yan E-mail:li_xyan@126.com

摘要:

目的 探讨中国汉族血脂异常2型糖尿病(T2DM)患者血浆总同型半胱氨酸(tHcy)水平与亚甲基四氢叶酸还原酶(MTHFR)基因C677T和A1298C多态性的相关性。
方法 研究纳入179例中国汉族T2DM患者,采用sanger双脱氧链终止法检测MTHFR C677T和A1298C的基因多态性,采用酶循环法检测血浆tHcy。按照血脂水平将患者分为血脂正常组和异常组,采用卡方检验比较两组MTHFRC677T和A1298C多态性基因型频率和等位基因频率的差异,采用Student's t检验比较两组中携带不同基因型患者血浆tHcy水平的差异。
结果 研究纳入T2DM血脂异常患者82例,T2DM血脂正常患者94例。T2DM患者的MTHFR C677T多态性与tHcy水平之间存在显著相关性(t=2.27,P=0.02)。携带MTHFR 677 TT基因型的血脂异常患者的血浆tHcy水平显著高于携带CT+CC基因型患者(13.62±6.97比10.95±3.62 μmol/L,t=2.20,P=0.03);而对于血脂正常的患者,携带上述两个等位基因的患者的tHcy水平无显著差异(13.34±6.03比12.04±5.09 μmol/L,t=1.08,P=0.29)。
结论 MTHFR 677TT基因多态性可能与血脂异常的2型糖尿病患者的血浆tHcy水平升高有关。

关键词: 2型糖尿病, 亚甲基四氢叶酸还原酶, 多态性, 同型半胱氨酸, 高脂血症

Abstract:

Objective To investigate the association between total homocysteine (tHcy) level in plasma and methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C genetic polymorphisms in a Chinese Han nationality population with type 2 diabetes mellitus (T2DM) accompanied by dyslipidemia.
Methods This case-control study enrolled T2DM patients with dyslipidemia and without dyslipidemia respectively. Sanger dideoxy-mediated chain-termination method was used to detect the gene polymorphisms of MTHFR C677T and A1298C. Plasma tHcy and lipid levels were measured as well. The genotype frequency and allele frequency between the dyslipidemia and non-dyslipidemia groups were compared by using Chi-square test. Plasma tHcy level of T2DM patients who carried the different genotypes was compared by Student’s t test.
Results Finally, 82 T2DM patients with dyslipidemia and 94 ones without dyslipidemia were included in this study. There was a significant correlation between tHcy level and MTHFR C677T gene polymorphism in T2DM patients (t=2.27, P=0.02). Moreover, the plasma tHcy level in the dyslipidemia patients who carried MTHFR 677 TT genotype was significantly higher than that in those with CT+CC genotype (13.62±6.97 vs. 10.95±3.62 μmol/L, t=2.20, P=0.03); while for patients without dyslipidemia, comparison of the tHcy level between those who carried the above two alleles showed no significantly difference (13.34±6.03 vs. 12.04±5.09 μmol/L, t=1.08, P=0.29).
Conclusion MTHFR 677TT genotype might associate with higher tHcy level in T2DM patients with dyslipidemia.

Key words: type 2 diabetes mellitus, methylenetetrahydrofolate reductase, polymorphism, homocysteine, hyperlipidemia

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