Endothelial nitric oxide synthase gene polymorphisms associated with susceptibility to high altitude pulmonary edema in Chinese railway construction workers at Qinghai-Tibet over 4 500 meters above sea Level

doi:10.1016/S1001-9294(11)60005-9

Chinese Medical Sciences Journal ›› 2010, Vol. 25 ›› Issue (4): 215-222.doi: 10.1016/S1001-9294(11)60005-9

Endothelial nitric oxide synthase gene polymorphisms associated with susceptibility to high altitude pulmonary edema in Chinese railway construction workers at Qinghai-Tibet over 4 500 meters above sea Level

Yu-jing Sun¹, Ming-wu Fang¹, Wen-quan Niu¹, Guang-ping Li¹, Jing-liang Liu², Shou-quan Ding², Ying Xu², Guo-shu Yu², Jian-qun Dong¹, Yun-jun Pan², Wei-ya Dong², Tian Wang², Jing-wen Cao², Xiao-bo Li², Zhong-xiang Wang², Guang-xue Yu², Hui-cheng Sun², Zhong-hou Jia², Jun Liu², Xiao-ming Wang¹, Qin Si¹, Qi-xia Wu¹, Wen-yu Zhou¹, Tong-chun Zhu², and Chang-chun Qiu¹

¹National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
²Hospital of China Railway Construction Corporation, Beijing 100039, China

出版日期:2010-12-20 发布日期:2010-12-20

Endothelial nitric oxide synthase gene polymorphisms associated with susceptibility to high altitude pulmonary edema in Chinese railway construction workers at Qinghai-Tibet over 4 500 meters above sea Level

Yu-jing Sun¹, Ming-wu Fang¹, Wen-quanNiu¹, Guang-ping Li¹, Jing-liang Liu², Shou-quanDing², Ying Xu², Guo-shu Yu², Jian-qun Dong¹,Yun-jun Pan²,Wei-ya Dong², Tian Wang², Jing-wenCao², Xiao-bo Li², Zhong-xiang Wang², Guang-xueYu², Hui-cheng Sun², Zhong-hou Jia², Jun Liu²,Xiao-ming Wang¹,Qin Si¹, Qi-xia Wu¹, Wen-yuZhou¹, Tong-chun Zhu², and Chang-chun Qiu¹*

¹National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
²Hospital of China Railway Construction Corporation, Beijing 100039, China

Online:2010-12-20 Published:2010-12-20

摘要/Abstract

摘要： Objective To examine whether the polymorphisms of endothelial nitric oxide synthase (eNOS) gene are associated with the susceptibility to high altitude pulmonary edema (HAPE) in Chinese railway construction workers at Qinghai-Tibet where the altitude is over 4 500 m above sea level. Methods A case-control study was conducted including 149 HAPE patients in the construction workers and 160 healthy controls randomly recruited from their co-workers, matching the patients in ethnicity, age, sex, lifestyle, and working conditions. Three polymorphisms of eNOS gene, T-786C in promoter, 894G/T in exon 7, and 27bp variable number tandem repeat (VNTR) in intron 4, were genotyped using polymerase chain reaction (PCR) and confirmed with DNA sequencing. Results The frequencies of 894T allele and heterozygous G/T of the 894G/T variant were significantly higher in HAPE patients group than in the control group (P=0.0028 and P=0.0047, respectively). However, the frequencies of the T-786C in promoter and the 27bp VNTR in intron 4 were not significantly different between the two groups. Haplotypic analysis revealed that the frequencies of two haplotypes (H3,T-T-b, b indicates 5 repeats of 27 bp VNTR; H6, C-G-a, a indicates 4 repeats of 27 bp VNTR) were significantly higher in HAPE patients (both P＜0.0001). On the contrary, the frequencies of H1 (T-G-b) and H2 (T-G-a) were lower in HAPE patients than in healthy controls (both P＜0.001). Conclusions Two haplotypes (T-T-b and C-G-a) may be strongly associated with susceptibility to HAPE. Compared with the individual alleles of eNOS gene, the interaction of multiple genetic markers within a haplotype may be a major determinant for the susceptibility to HAPE.

关键词: high altitude pulmonary edema, nitric oxide synthase;gene polymorphism, haplotype

Abstract: Objective To examine whether the polymorphisms of endothelial nitric oxide synthase (eNOS) gene are associated with the susceptibility to high altitude pulmonary edema (HAPE) in Chinese railway construction workers at Qinghai-Tibet where the altitude is over 4 500 m above sea level. Methods A case-control study was conducted including 149 HAPE patients in the construction workers and 160 healthy controls randomly recruited from their co-workers, matching the patients in ethnicity, age, sex, lifestyle, and working conditions. Three polymorphisms of eNOS gene, T-786C in promoter, 894G/T in exon 7, and 27bp variable number tandem repeat (VNTR) in intron 4, were genotyped using polymerase chain reaction (PCR) and confirmed with DNA sequencing. Results The frequencies of 894T allele and heterozygous G/T of the 894G/T variant were significantly higher in HAPE patients group than in the control group (P=0.0028 and P=0.0047, respectively). However, the frequencies of the T-786C in promoter and the 27bp VNTR in intron 4 were not significantly different between the two groups. Haplotypic analysis revealed that the frequencies of two haplotypes (H3,T-T-b, b indicates 5 repeats of 27 bp VNTR; H6, C-G-a, a indicates 4 repeats of 27 bp VNTR) were significantly higher in HAPE patients (both P＜0.0001). On the contrary, the frequencies of H1 (T-G-b) and H2 (T-G-a) were lower in HAPE patients than in healthy controls (both P＜0.001). Conclusions Two haplotypes (T-T-b and C-G-a) may be strongly associated with susceptibility to HAPE. Compared with the individual alleles of eNOS gene, the interaction of multiple genetic markers within a haplotype may be a major determinant for the susceptibility to HAPE.

Key words: high altitude pulmonary edema, nitric oxide synthase;gene polymorphism, haplotype

Yu-jing Sun, Ming-wu Fang, Wen-quan Niu, Guang-ping Li, Jing-liang Liu, Shou-quan Ding, Ying Xu, Guo-shu Yu, Jian-qun Dong, Yun-jun Pan,Wei-ya Dong, Tian Wang, Jing-wen Cao, Xiao-bo Li, Zhong-xiang Wang, Guang-xue Yu, Hui-cheng Sun, Zhong-hou Jia, Jun Liu, Xiao-ming Wang, Qin Si, Qi-xia Wu, Wen-yu Zhou, Tong-chun Zhu and Chang-chun Qiu. Endothelial nitric oxide synthase gene polymorphisms associated with susceptibility to high altitude pulmonary edema in Chinese railway construction workers at Qinghai-Tibet over 4 500 meters above sea Level[J].Chinese Medical Sciences Journal, 2010, 25(4): 215-222.

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Endothelial nitric oxide synthase gene polymorphisms associated with susceptibility to high altitude pulmonary edema in Chinese railway construction workers at Qinghai-Tibet over 4 500 meters above sea Level

Endothelial nitric oxide synthase gene polymorphisms associated with susceptibility to high altitude pulmonary edema in Chinese railway construction workers at Qinghai-Tibet over 4 500 meters above sea Level

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