检测淀粉样沉淀蛋白的三种染色方法比较：抗体染色、Gallyas银染和硫磺素S染色

doi:10.24920/03476

摘要/Abstract

摘要：

目的探究阿尔茨海默病小鼠模型的淀粉样沉淀的形成过程。比较抗体染色,Gallyas银染和硫磺素S染色3种染色技术检测淀粉样沉淀的效果。方法检测不同月龄的APPswe/PS1dE9转基因小鼠（APP/PS1）大脑淀粉样沉积的时空变化。对小鼠同一脑区的临近切片行抗体染色,Gallyas银染和硫磺素S染色,并对比其染色效果。结果随着模型鼠年龄的增大,淀粉样沉积首先出现在的大脑皮层,随后扩散至整个大脑。与Gallyas银染和硫磺素S染色比较,6E10抗体染色能检测到更多的淀粉样斑块沉积（P<0.05）。Gallyas银染和硫磺素S染色的检测结果之间没有显著的统计学意义（P = 0.0033）。结论 APP/PS1转基因小鼠模型能够模拟阿尔茨海默病患者脑内的淀粉样沉积过程。抗体染色得到的结果相较于其它两种检测方法更为可靠。

关键词: 淀粉样沉淀, 阿尔茨海默病, 抗体染色, Gallyas银染, 硫磺素S染色

Abstract:

Objective To evaluate senile plaque formation and compare the sensitivity of three different β-amyloid (Aβ) labeling methods (antibody staining, Gallyas silver staining, and thioflavin-S staining) to detect Aβ deposition.Methods APPswe/PSEN1dE9 transgenic mice (APP/PS1) of different ages were used to examine spatiotemporal changes in Aβ plaque deposition. Antibody staining, Gallyas silver staining, and thioflavin-S staining were used to detect Aβ plaque deposition in the same brain region of adjacent slices from model mice, and the results were compared.Results With aging, Aβ plaques first appeared in the cortex and then the deposition increased throughout the whole brain. Significantly greater plaque deposition was detected by 6E10 antibody than that analyzed with Gallyas silver staining or thioflavin-S staining (P<0.05). Plaque deposition did not show significant difference between the APP/PS1 mice brains assayed with Gallyas silver staining and ones with thioflavin-S staining (P=0.0033).Conclusions The APP/PS1 mouse model of Alzheimer’s disease could mimick the progress of Aβ plaques occurred in patients with Alzheimer’s disease. Antibody detection of Aβ deposition may be more sensitive than chemical staining methods.

Key words: β-amyloid plaques, Alzheimer’s disease, antibody staining, Gallyas silver, thioflavin-S

Shi Xinze, Wei Xuan, Sha Longze, Xu Qi. Comparison of β-Amyloid Plaque Labeling Methods: Antibody Staining, Gallyas Silver Staining, and Thioflavin-S Staining[J].Chinese Medical Sciences Journal, 2018, 33(3): 167-173.

图/表 4

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