Gaussia Luciferase Reporter Assay for Assessment of Gene Delivery Systems in Vivo

doi:10.1016/S1001-9294(10)60029-6

Chinese Medical Sciences Journal ›› 2010, Vol. 25 ›› Issue (2): 95-99.doi: 10.1016/S1001-9294(10)60029-6

• Original Article • 上一篇下一篇

Gaussia Luciferase Reporter Assay for Assessment of Gene Delivery Systems in Vivo

Feng Chen, Zhen Xu, Jing Lu, Xiang Lv, Wen-li Mu, Ya-jun Wang,De-pei Liu*, and Chih-chuan Liang

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China

收稿日期:2010-05-07 出版日期:2010-06-10 发布日期:2010-06-10
作者简介:Tel: 86-10-65296415, Fax: 86-10-65105093

Gaussia Luciferase Reporter Assay for Assessment of Gene Delivery Systems in Vivo

Feng Chen, Zhen Xu, Jing Lu, Xiang Lv, Wen-li Mu, Ya-jun Wang,De-pei Liu*, and Chih-chuan Liang

National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China

Received:2010-05-07 Online:2010-06-10 Published:2010-06-10
About author:Tel: 86-10-65296415, Fax: 86-10-65105093

摘要/Abstract

摘要： Objective To develop an alternative method for assessment of gene delivery systems in vivo. Methods Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase (Gluc) expression cassette. After implantation of these cells into recipient mice, the expression of Gluc was detected in whole blood or plasma collected. Results As little as 10 uL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer. And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice. Conclusions Gluc may be useful as an in vivo reporter for gene therapy researches, and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.

关键词: gene delivery systems, reporter, Gaussia luciferase

Abstract: Objective To develop an alternative method for assessment of gene delivery systems in vivo. Methods Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase (Gluc) expression cassette. After implantation of these cells into recipient mice, the expression of Gluc was detected in whole blood or plasma collected. Results As little as 10 uL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer. And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice. Conclusions Gluc may be useful as an in vivo reporter for gene therapy researches, and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.

Key words: gene delivery systems, reporter, Gaussia luciferase

基金资助:

Supported by National High Technology Research and De- velopment Program of China (863 Program) (2007AA021206, 2007AA021106)

Feng Chen, Zhen Xu, Jing Lu, Xiang Lü, Wen-li Mu, Ya-jun Wang, De-pei Liu and Chih-chuan Liang. Gaussia Luciferase Reporter Assay for Assessment of Gene Delivery Systems in Vivo[J].Chinese Medical Sciences Journal, 2010, 25(2): 95-99.

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