Chinese Medical Sciences Journal ›› 2012, Vol. 27 ›› Issue (3): 171-175.doi: 10.1016/S1001-9294(14)60051-1

• Original Article • Previous Articles     Next Articles

Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△

Li-shan Lian#, Yao-guo Yang#, Wei Liu, Li-long Guo, Heng Guan, Chang-wei Liu, Yong-jun Li*   

  1. Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China
  • Received:2012-03-11 Revised:2012-10-08 Online:2012-10-08 Published:2012-10-08
  • About author:#These two authors equally contribute to this work.*Corresponding author Tel: 86-10- 69158230, E-mail: Yongj_93@ gmail.com
  • Supported by:

    △Supported by the National Natural Science Foundation of China (81270399 and 81100226).

Abstract: To construct the zinc finger protein-activating transcription factor (ZFP-ATF) plasmid and evaluate its efficacy in inducing vascular endothelial growth factor (VEGF) expression in EY.HY926 endothelial cells.
Methods Firstly, we constructed the ZFP-ATF plasmid, then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot, finally, we used the RT-PCR to testify whether the ZFP-ATF can stimulate expression of VEGF splice variants.
Results The ZFP-ATF DNA sequences were located the multiclone sites of PVAX1 vector between the site of BamHⅠand Xhol.Western blot result showed VEGF expression in EY.HY926 endothelial cells transfected with ZFP-ATF plasmid was significantly higher than that in cells transfected with VEGF165 (19.95±3.95 vs.12.15±1.55 μg/μL, P<0.01).RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165.
Conclusion ZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165, which might have beneficial effects in angiogenesis process.

Key words: zinc finger protein-activating transcription factor, vascular endothelial growth factor, angiogenesis

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