Chinese Medical Sciences Journal ›› 2019, Vol. 34 ›› Issue (3): 184-193.doi: 10.24920/003558

• Original Article • Previous Articles     Next Articles

Downregulation of iASPP Expression Suppresses Proliferation, Invasion and Increases Chemosensitivity to Paclitaxel of Head and Neck Squamous Cell Carcinoma In Vitro

Liu Zhengzheng1, *(), Kuang Weilu1, Zeng Wenjing3, Xiao Jianyun2, Tian Yongquan2, *()   

  1. 1 Department of Oncology, , Xiangya Hospital, Central South University, Changsha 410008, China
    2 Department of Pharmacy, , Xiangya Hospital, Central South University, Changsha 410008, China
    3 Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha 410008, China
  • Received:2019-07-02 Online:2019-09-30 Published:2019-10-11
  • Contact: Liu Zhengzheng,Tian Yongquan E-mail:lzzsmile@aliyun.com;tianyongquan@aliyun.com
  • Supported by:
    Supported by Beijing Medical Health Public Welfare Foundation(grant no. YWJKJJHKYJJ-B17468 and running period: 2017.09.01-2020.09.01)

Abstract:

Objective Our previous study has revealed that iASPP is elevated in human head and neck squamous cell carcinoma (HNSCC) and iASPP overexpression signifcantly correlates with tumor malignant progression and poor survival of HNSCC. This study investigated the function of iASPP playing in proliferation and invasion of HNSCC in vitro.

Methods HNSCC cell line Tu686 transfected with Lentiviral vector-mediated iASPP-specific shRNA and control shRNA were named the shRNA-iASPP group and shRNA-NC group, respectively. The non-infected Tu686 cells were named the CON group. CCK-8 assay, flow cytometry, transwell invasion assay were performed to detect the effects of iASPP inhibition in vitro.

Results Our results demonstrated that the proliferation of shRNA-iASPP cells at the time of 72 h (F=32.459, P=0.000), 96 h (F=51.407, P=0.000), 120 h (F=35.125, P=0.000) post-transfection, was significantly lower than that of shRNA-NC cells and CON cells. The apoptosis ratio of shRNA-iASPP cells was 9.42% ± 0.39% (F=299.490, P=0.000), which was significantly higher than that of CON cells (2.80% ± 0.42%) and shRNA-NC cells (3.18% ± 0.28%). The percentage of shRNA-iASPP cells in G0/G1 phase was 74.65% ± 1.09% (F=388.901, P=0.000), which was strikingly increased, compared with that of CON cells (55.19% ± 1.02%) and shRNA-NC cells (54.62% ± 0.88%). The number of invading cells was 56 ± 4 in the shRNA-iASPP group (F=84.965, P=0.000), which decreased significantly, compared with the CON group (111 ± 3) and the shRNA-NC group (105 ± 8). The survival rate of shRNA-iASPP cells administrated with paclitaxel was highly decreased, compared with CON cells and shRNA-NC cells (F=634.841, P=0.000).

Conclusion These results suggest iASPP may play an important role in progression and aggressive behavior of HNSCC and may be an efficient chemotherapeutic target for the treatment of HNSCC.

Key words: human head and neck squamous cell carcinoma, iASPP, chemosensitivity, paclitaxel

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