Chinese Medical Sciences Journal ›› 2022, Vol. 37 ›› Issue (1): 44-51.doi: 10.24920/003889
• Original Article • Previous Articles Next Articles
Evaluation of the Gastric Microbiome in Patients with Chronic Superficial Gastritis and Intestinal Metaplasia
Ying Liu1, *(
), Yongjun Ma1, Caiqun Huang2
- 1Department of Clinical Laboratory, Jinhua Municipal Central Hospital, Jinhua, Zhejiang, 321000, China
2Department of Central Laboratory, Jinhua Municipal Central Hospital, Jinhua, Zhejiang, 321000, China
-
Received:2021-02-18Accepted:2021-09-18Published:2022-03-31Online:2022-03-04 -
Contact:Ying Liu E-mail:liuyingtj2008@sina.com
Cite this article
Ying Liu, Yongjun Ma, Caiqun Huang. Evaluation of the Gastric Microbiome in Patients with Chronic Superficial Gastritis and Intestinal Metaplasia[J].Chinese Medical Sciences Journal, 2022, 37(1): 44-51.
share this article
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
Table 1
Clinical characteristics of patients with chronic superficial gastritis or intestinal metaplasia (n=54)"
| Group | Age (years, mean±SD) | t/χ2 | P value | Sex /male [n(%)] | t/χ2 | P value |
|---|---|---|---|---|---|---|
| G-HN (n=24) | 55.7±13.7 | -1.275 | 0.210 | 9(37.5) | 1.380 | 0.318 |
| G-HP (n=14) | 50.8±5.4 | 8(57.1) | ||||
| I-HN (n=11) | 60.5±9.9 | -1.162 | 0.265 | 7(63.6) | 2.424 | 0.245 |
| I-HP (n=5) | 54.6±8.4 | 5(100) |
Table 1
Figure 1.
Alpha and beta diversity analyses. (A) Measurement of alpha diversity through rarefaction curves for observed species. (B) Shannon’s index (Logistic regression adjusted for age and sex). (C) Simpson’s index (Logistic regression adjusted for age and sex). (D) 2D-PCoA of distances of 16S rRNA genes. (E) ANOSIM revealed statistically significant difference (P=0.001)."
Figure 1.
Figure 2.
Bacterial compositions among four groups. (A) Bacterial compositions at the phylum level. (B) Bacterial compositions at the genus level. (C) Venn diagram of the phylum level. (D) Venn diagram of the genus level."
Figure 2.
Figure 3.
Analysis for bacterial abundance of the four groups. (A) Bar graph (error bar) for bacterial abundance. Each column represents the relative abundance of a species. The differential bacterial genera among the four groups are presented in red letters. (B) Classification tree heat map for bacterial abundance. The orange balls represent a significant difference (q value) among the four groups. The middle layer of red and white is the average abundance heat map. A darker color represents greater abundance. The out layer is the annotated species."
Figure 3.
Table 2
Comparison of bacterial genera in G-HP and I-HP groups [%]§"
| Group | Neisseria | Streptococcus | Rothia | Veillonella |
|---|---|---|---|---|
| G-HP(%) (mean±SD) | 0.5±0.01 | 0.5±0.00 | 0.1±0.00 | 0.3±0.01 |
| I-HP (%) (mean±SD) | 13.8±0.30 | 1.7±0.02 | 1.5±0.03 | 1.4±0.03 |
| t | 175.1 | 138.5 | 184.7 | 124.4 |
| P-value | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
Table 2
| 1. |
Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984; 1:1311-5. doi: 10.1016/s0140-6736(84)91816-6.
doi: 10.1016/s0140-6736(84)91816-6 pmid: 6145023 |
| 2. |
Ianiro G, Molina-Infante J, Gasbarrini A. Gastric Microbiota. Helicobacter 2015; 20(Suppl 1):68-71. doi: 10.1111/hel.12260.
doi: 10.1111/hel.12260 |
| 3. |
Leja M, Grinberga-Derica I, Bilgilier C, et al. Review: Epidemiology of Helicobacter pylori infection. Helicobacter 2019; 24(Suppl 1):e12635. doi: 10.1111/hel.12635.
doi: 10.1111/hel.12635 |
| 4. |
Polk DB, Peek RM Jr. Helicobacter pylori: Gastric cancer and beyond. Nat Rev Cancer 2010; 10(6):403-14. doi: 10.1038/nrc2857.
doi: 10.1038/nrc2857 |
| 5. |
Dias-Jácome E, Libânio D, Borges-Canha M, et al. Gastric microbiota and carcinogenesis: the role of non-Helicobacter pylori bacteria - A systematic review. Rev Esp Enferm Dig 2016; 108(9):530-40. doi: 10.17235/reed.2016.4261/2016.
doi: 10.17235/reed.2016.4261/2016 pmid: 27604361 |
| 6. |
Uemura N, Okamoto S, Yamamoto S, et al. Helicobacter pylori infection and the development of gastric cancer. N Engl J Med 2001; 345(11):784-9. doi: 10.1056/NEJMoa001999.
doi: 10.1056/NEJMoa001999 |
| 7. |
Ma JL, Zhang L, Brown LM, et al. Fifteen-year effects of Helicobacter pylori, garlic, and vitamin treatments on gastric cancer incidence and mortality. J Natl Cancer Inst 2012; 104:488-92. doi: 10.1093/jnci/djs003.
doi: 10.1093/jnci/djs003 |
| 8. |
Li WQ, Ma JL, Zhang L, et al. Effects of Helicobacter pylori treatment on gastric cancer incidence and mortality in subgroups. J Natl Cancer Inst 2014; 106(7):dju116. doi: 10.1093/jnci/dju116.
doi: 10.1093/jnci/dju116 |
| 9. |
Wong BC, Zhang L, Ma JL, et al. Effects of selective COX-2 inhibitor and Helicobacter pylori eradication on precancerous gastric lesions. Gut 2012; 61(6):812-8. doi: 10.1136/gutjnl-2011-300154.
doi: 10.1136/gutjnl-2011-300154 |
| 10. | International Agency for Research on Cancer. Helicobacter pylori eradication as a strategy for preventing gastric cancer. IARC working group reports Volume 8. Available from https://www.gastro-health-now.org/wp/wp-content/uploads/2014/09/WHO-IARC-Report-2014.pdf. accessed: January 1. |
| 11. |
Pohl D, Keller PM, Bordier V, et al. Review of current diagnostic methods and advances in Helicobacter pylori diagnostics in the era of next generation sequencing. World J Gastroenterol 2019; 25(32):4629-4660. doi: 10.3748/wjg.v25.i32.4629.
doi: 10.3748/wjg.v25.i32.4629 |
| 12. |
Zhao Y, Gao X, Guo J, et al. Helicobacter pylori infection alters gastric and tongue coating microbial communities. Helicobacter. 2019; 24(2):e12567. doi: 10.1111/hel.12567.
doi: 10.1111/hel.12567 |
| 13. |
Gantuya B, El Serag HB, Saruuljavkhlan B, et al. Advantage of 16S rRNA amplicon sequencing in Helicobacter pylori diagnosis. Helicobacter 2021; 26(3):e12790. doi: 10.1111/hel.12790.
doi: 10.1111/hel.12790 |
| 14. |
Park CH, Lee AR, Lee YR, et al. Evaluation of gastric microbiome and metagenomic function in patients with intestinal metaplasia using 16S rRNA gene sequencing. Helicobacter 2019; 24(1):e12547. doi: 10.1111/hel.12547.
doi: 10.1111/hel.12547 |
| 15. |
Schulz C, Schütte K, Koch N, et al. The active bacterial assemblages of the upper GI tract in individuals with and without Helicobacter infection. Gut 2018; 67(2):216-25. doi: 10.1136/gutjnl-2016-312904.
doi: 10.1136/gutjnl-2016-312904 |
| 16. |
Parsons BN, Ijaz UZ, D’Amore R, et al. Comparison of the human gastric microbiota in hypochlorhydric states arising as a result of Helicobacter pylori-induced atrophic gastritis, autoimmune atrophic gastritis and proton pump inhibitor use. PLoS Pathog 2017; 13(11):e1006653. doi: 10.1371/journal.
doi: 10.1371/journal |
| 17. |
Klymiuk I, Bilgilier C, Stadlmann A, et al. The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori. Front Microbiol 2017; 8:2508. doi: 10.3389/fmicb.2017.02508.
doi: 10.3389/fmicb.2017.02508 |
| 18. |
Ahn J, Sinha R, Pei Z, et al. Human gut microbiome and risk for colorectal cancer. J Natl Cancer Inst 2013; 105(24):1907-11. doi: 10.1093/jnci/djt300.
doi: 10.1093/jnci/djt300 |
| 19. |
Coker OO, Dai Z, Nie Y, et al. Mucosal microbiome dysbiosis in gastric carcinogenesis. Gut 2018; 67(6):1024-32. doi: 10.1136/gutjnl-2017-314281.
doi: 10.1136/gutjnl-2017-314281 |
| 20. |
Ferreira RM, Pereira-Marques J, Pinto-Ribeiro I, et al. Gastric microbial community profiling reveals a dysbiotic cancer-associated microbiota. Gut 2018; 67(2):226-36. doi: 10.1136/gutjnl-2017-314205.
doi: 10.1136/gutjnl-2017-314205 pmid: 29102920 |
| 21. |
Palrasu M, Zaika E, El-Rifai W, et al. Role of Bacterial and Viral Pathogens in Gastric Carcinogenesis. Cancers (Basel) 2021; 13(8):1878. doi: 10.3390/cancers13081878.
doi: 10.3390/cancers13081878 |
| 22. |
Song H, Ekheden IG, Zheng Z, et al. Incidence of gastric cancer among patients with gastric precancerous lesions: observational cohort study in a low risk Western population. BMJ 2015; 351:h3867. doi: 10.1136/bmj.h3867.
doi: 10.1136/bmj.h3867 |
| 23. |
Amieva M, Peek RM Jr. Athobiology of helicobacter pylori-induced gastric cancer. Gastroenterology 2016; 150(1):64-78. doi: 10.1053/j.gastro.2015.09.004.
doi: 10.1053/j.gastro.2015.09.004 |
| 24. |
Li F, Zhu H, Tao K, et al. Mucosal microbial microenvironment in early gastric neoplasia and non-neoplastic gastric disease. J Gastroenterol Hepatol 2021; 36(11):3092-101. doi: 10.1111/jgh.15565.
doi: 10.1111/jgh.15565 |
| 25. |
Guo Y, Zhang Y, Gerhard M, et al. Effect of Helicobacter pylori on gastrointestinal microbiota: a population-based study in Linqu, a high-risk area of gastric cancer. Gut 2020; 69(9):1598-607. doi: 10.1136/gutjnl-2019-319696.
doi: 10.1136/gutjnl-2019-319696 |
| No related articles found! |
| Viewed | ||||||
|
Full text |
|
|||||
|
Abstract |
|
|||||
|
