Chinese Medical Sciences Journal ›› 2022, Vol. 37 ›› Issue (4): 331-339.doi: 10.24920/004006

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Topoisomerase α Gene as a Marker for Prognostic Prediction of Hepatocellular Carcinoma: A Bioinformatics Analysis

Jin Lu1, 2, Shaoguang An3, Junjie Ma3, Yue Yang4, Lei Zhang5, Peng Yu1, Heng Tao1, Yunfan Chen1, Haoxuan Zhang1, 2, *()   

  1. 1School of Basic Medicine, Bengbu Medical College, Bengbu, Anhui 233030, China
    2Anhui Key Laboratory of Computational Medicine and Intelligent Health, Bengbu Medical College, Bengbu, Anhui 233030, China
    3Clinical Medical College, Bengbu Medical College, Bengbu, Anhui 233030, China
    4Department of Otolaryngology, First Affiliated Hospital, Bengbu Medical College, Bengbu, Anhui 233030, China
    5Department of Surgical Oncology, Second Affiliated Hospital, Bengbu Medical College, Bengbu, Anhui 233030, China
  • Received:2021-09-22 Accepted:2021-12-10 Published:2022-12-31 Online:2022-10-04
  • Contact: Haoxuan Zhang E-mail:0100197@bbmc.edu.cn

Objective To investigate the expression of topoisomeraseα (TOP2α) in hepatocellular carcinoma (HCC) and its role in predicting prognosis of HCC patients.
Methods We used HCC-related datasets in UALCAN, HCCDB, and cBioPortal databases to analyze the expression and mutation of TOP2α and its co-expressed genes in HCC tissues. GO function and KEGG pathway enrichment of TOP2α and its co-expressed genes were identified. The TIMER database was used to analyze infiltration levels of immune cells in HCC. The impacts of TOP2α and its co-expression genes and the infiltrated immune cells on the survival of HCC patients were assayed by Kaplan-Meier plotter analysis.
Results TOP2α and its co-expression genes were highly expressed in HCC (P< 0.001) and detrimental to overall survival of HCC patients (P< 0.001). TOP2α and its co-expression genes were mainly involved in cell mitosis and proliferation, and cell cycle pathway (ID: hsa04110, P = 0.001945). TOP2α and its co-expression genes were mutated in HCC and the mutations were significantly detrimental to overall survival (P = 0.0247) and disease-free survival (P = 0.0265) of HCC patients. High TOP2α expression was positively correlated with the infiltration of B cell (r = 0.459, P< 0.01), CD8+ T cell (r = 0.312, P< 0.01), CD4+ T cell (r = 0.370, P< 0.01), macrophage (r = 0.459, P< 0.01), neutrophil (r = 0.405, P< 0.01), and dendritic cell (r = 0.473, P< 0.01) in HCC. The CD8+ T cell infiltration significantly prolonged the 3- and 5-year survival of HCC patients (all P< 0.05), and CD4+ T cell infiltration significantly shortened the 3-, 5-, and 10-year survival of HCC patients (all P< 0.05).
Conclusion TOP2α may be an oncogene, which was associated with poor prognosis of HCC patients and could be used as a biomarker for the prognostic prediction of HCC.

Key words: topoisomeraseⅡα, disease-free survival, overall survival, hepatocellular carcinoma, bioinformatics analysis

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