Chinese Medical Sciences Journal ›› 2022, Vol. 37 ›› Issue (4): 331-339.doi: 10.24920/004006
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Jin Lu1, 2, Shaoguang An3, Junjie Ma3, Yue Yang4, Lei Zhang5, Peng Yu1, Heng Tao1, Yunfan Chen1, Haoxuan Zhang1, 2, *()
Received:
2021-09-22
Accepted:
2021-12-10
Published:
2022-12-31
Online:
2022-10-04
Contact:
Haoxuan Zhang
E-mail:0100197@bbmc.edu.cn
Jin Lu, Shaoguang An, Junjie Ma, Yue Yang, Lei Zhang, Peng Yu, Heng Tao, Yunfan Chen, Haoxuan Zhang. Topoisomerase Ⅱα Gene as a Marker for Prognostic Prediction of Hepatocellular Carcinoma: A Bioinformatics Analysis[J].Chinese Medical Sciences Journal, 2022, 37(4): 331-339.
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Figure 1.
Expression of TOP2α in HCC and its effect on survival of HCC patients. (A) Comparison of expression of TOP2α gene in HCC and normal liver tissues based on the Cancer Genome Atlas (TCGA) database (P < 0.001). (B) Effect of TOP2α high expression on OS of HCC patients. TOP2α: topoisomerase Ⅱ α; HCC: hepatocellular carcinoma; OS: overall survival."
Table 1.
Correlation between TOP2α and co-expression genes in HCC"
Co-expression genes | TOP2α coeffcient | ||
---|---|---|---|
Log2OR | P value | Spearman correlation | |
KIF18B | >3 | <0.001 | 0.95 |
BUB1B | >3 | <0.001 | 0.94 |
CKAP2L | >3 | <0.001 | 0.93 |
ANLN | >3 | <0.001 | 0.93 |
TPX2 | >3 | <0.001 | 0.93 |
BUB1 | >3 | <0.001 | 0.92 |
PRC1 | >3 | <0.001 | 0.92 |
NUSAP1 | >3 | <0.001 | 0.92 |
KIF11 | >3 | <0.001 | 0.92 |
GINS1 | >3 | <0.001 | 0.91 |
HJURP | >3 | <0.001 | 0.91 |
MELK | >3 | <0.001 | 0.90 |
TTK | >3 | <0.001 | 0.90 |
DLGAP5 | >3 | <0.001 | 0.90 |
KIFC1 | >3 | <0.001 | 0.90 |
KIF4A | >3 | <0.001 | 0.90 |
NCAPG | >3 | <0.001 | 0.90 |
KIF20A | >3 | <0.001 | 0.90 |
CCNB2 | >3 | <0.001 | 0.87 |
Table 2.
GO and KEGG pathway enrichment of TOP2α and the co-expression genes"
Category | Biological function | GO term ID | Adjusted P value |
---|---|---|---|
Biological process | Mitotic spindle organization | GO:0007052 | 4.30E-07 |
Mitotic sister chromatid segregation | GO:0000070 | 4.01E-07 | |
Mitotic nuclear division | GO:0140014 | 1.63E-05 | |
Sister chromatid segregation | GO:0000819 | 3.72E-05 | |
Microtubule cytoskeleton organization involved in mitosis | GO:1902850 | 1.08E-04 | |
Mitotic spindle assembly | GO:0090307 | 1.07E-04 | |
Mitotic cytokinesis | GO:0000281 | 2.49E-04 | |
Mitotic spindle assembly checkpoint | GO:0007094 | 3.68E-04 | |
Mitotic spindle checkpoint | GO:0071174 | 3.27E-04 | |
Spindle assembly checkpoint | GO:0071173 | 2.94E-04 | |
Cytoskeleton-dependent cytokinesis | GO:0061640 | 3.14E-04 | |
Chromosome condensation | GO:0030261 | 6.36E-04 | |
Centromeric sister chromatid cohesion | GO:0070601 | 0.005580 | |
Cellular component | Spindle | GO:0005819 | 3.04E-13 |
Microtubule cytoskeleton | GO:0015630 | 2.39E-10 | |
Mitotic spindle | GO:0072686 | 2.71E-08 | |
Microtubule | GO:0005874 | 1.01E-07 | |
Kinesin complex | GO:0005871 | 1.18E-07 | |
Spindle microtubule | GO:0005876 | 1.21E-05 | |
Condensed chromosome kinetochore | GO:0000777 | 5.23E-05 | |
Polymeric cytoskeletal fiber | GO:0099513 | 1.22E-04 | |
Condensed chromosome, centromeric region | GO:0000779 | 1.22E-04 | |
Condensed nuclear chromosome kinetochore | GO:0000778 | 0.002317 | |
Condensed nuclear chromosome, centromeric region | GO:0000780 | 0.002527 | |
Microtubule-organizing center | GO:0005815 | 0.004354 | |
Spindle pole | GO:0000922 | 0.005601 | |
Molecular function | Microtubule motor activity | GO:0003777 | 3.85E-06 |
Motor activity | GO:0003774 | 1.11E-05 | |
Microtubule-binding | GO:0008017 | 1.09E-05 | |
Tubulin-binding | GO:0015631 | 3.98E-05 | |
ATPase activity | GO:0016887 | 3.31E-04 | |
ATP-dependent microtubule motor activity, plus-end-directed | GO:0008574 | 7.99E-04 | |
ATP-dependent microtubule motor activity | GO:1990939 | 0.00251 | |
Nucleoside-triphosphatase activity | GO:0017111 | 0.00725 | |
KEGG | Cell cycle pathway | hsa04110 | 0.001945 |
Table 3.
Comparisons of expression of the 19 TOP2α co-expression genes between HCC tissues and normal liver tissues and their associations with OS of HCC"
Genes | Expression level | OS | |||||
---|---|---|---|---|---|---|---|
Normal liver | HCC | P value | HR | 95%CI | P value | ||
ANLN | Low | High | <0.001 | 2.38 | 1.68-3.38 | 5.90E-07 | |
BUB1 | Low | High | <0.001 | 2.11 | 1.49-2.99 | 1.60E-05 | |
BUB1B | Low | High | <0.001 | 2.10 | 1.46-3.03 | 4.30E-05 | |
CCNB2 | Low | High | < 0.001 | 1.89 | 1.31-2.71 | 0.00048 | |
CKAP2L | Low | High | <0.001 | 2.03 | 1.43-2.88 | 4.70E-05 | |
DLGAP5 | Low | High | <0.001 | 2.28 | 1.61-3.22 | 1.50E-06 | |
GINS1 | Low | High | <0.001 | 2.36 | 1.64-2.37 | 1.50E-06 | |
HJURP | Low | High | <0.001 | 2.09 | 1.48-2.95 | 2.00E-05 | |
KIF11 | Low | High | <0.001 | 2.35 | 1.64-3.36 | 1.70E-06 | |
KIF18B | Low | High | <0.001 | 2.20 | 1.54-3.16 | 1.10E-05 | |
KIF20A | Low | High | <0.001 | 2.46 | 1.72-3.51 | 3.30E-07 | |
KIF4A | Low | High | <0.001 | 2.17 | 1.52-3.10 | 1.30E-05 | |
KIFC1 | Low | High | <0.001 | 2.53 | 1.78-3.58 | 7.10E-08 | |
MELK | Low | High | <0.001 | 2.38 | 1.68-3.37 | 4.90E-07 | |
NCAPG | Low | High | <0.001 | 2.20 | 1.53-3.17 | 1.20E-05 | |
NUSAP1 | Low | High | <0.001 | 1.77 | 1.25-2.50 | 0.0012 | |
PRC1 | Low | High | <0.001 | 1.91 | 1.35-2.70 | 0.00021 | |
TPX2 | Low | High | <0.001 | 2.32 | 1.64-3.27 | 9.30E-07 | |
TTK | Low | High | <0.001 | 2.82 | 1.96-4.07 | 6.50E-09 |
Figure 5.
Effect of mutation type on infiltration of immune cells in HCC. **P < 0.01, ***P < 0.001, TIMER database analysis showed when HCC patients with high amplication of TOP2α, the infiltration level of immune cells in the HCC immune microenvironment was significantly lower than that of patients carrying other mutations."
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