Chinese Medical Sciences Journal ›› 2018, Vol. 33 ›› Issue (3): 167-173.doi: 10.24920/03476

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Comparison of β-Amyloid Plaque Labeling Methods: Antibody Staining, Gallyas Silver Staining, and Thioflavin-S Staining

Shi Xinze1, Wei Xuan2, Sha Longze1, Xu Qi1, *()   

  1. 1 State Key Laboratory of Medical Molecular Biology and Neuroscience Center, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & School of Basic Medicine Peking Union Medical College, Beijing 100005, China;
    2 High School Attached to Beijing University of Technology,Beijing 100022, China
  • Online:2018-09-30 Published:2018-09-28
  • Contact: Xu Qi
  • Supported by:
    Supported by the 2016 Major Collaborative Innovation Program of the Chinese Academy of Medical Sciences(2016-I2M-1004)


Objective To evaluate senile plaque formation and compare the sensitivity of three different β-amyloid (Aβ) labeling methods (antibody staining, Gallyas silver staining, and thioflavin-S staining) to detect Aβ deposition.Methods APPswe/PSEN1dE9 transgenic mice (APP/PS1) of different ages were used to examine spatiotemporal changes in Aβ plaque deposition. Antibody staining, Gallyas silver staining, and thioflavin-S staining were used to detect Aβ plaque deposition in the same brain region of adjacent slices from model mice, and the results were compared.Results With aging, Aβ plaques first appeared in the cortex and then the deposition increased throughout the whole brain. Significantly greater plaque deposition was detected by 6E10 antibody than that analyzed with Gallyas silver staining or thioflavin-S staining (P<0.05). Plaque deposition did not show significant difference between the APP/PS1 mice brains assayed with Gallyas silver staining and ones with thioflavin-S staining (P=0.0033).Conclusions The APP/PS1 mouse model of Alzheimer’s disease could mimick the progress of Aβ plaques occurred in patients with Alzheimer’s disease. Antibody detection of Aβ deposition may be more sensitive than chemical staining methods.

Key words: β-amyloid plaques, Alzheimer’s disease, antibody staining, Gallyas silver, thioflavin-S

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