Chinese Medical Sciences Journal ›› 2018, Vol. 33 ›› Issue (3): 167-173.doi: 10.24920/03476
• Original Article • Previous Articles Next Articles
Shi Xinze1, Wei Xuan2, Sha Longze1, Xu Qi1, *()
Published:
2018-09-30
Online:
2018-09-28
Contact:
Xu Qi
E-mail:xuqi@pumc.edu.cn
Shi Xinze, Wei Xuan, Sha Longze, Xu Qi. Comparison of β-Amyloid Plaque Labeling Methods: Antibody Staining, Gallyas Silver Staining, and Thioflavin-S Staining[J].Chinese Medical Sciences Journal, 2018, 33(3): 167-173.
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Figure 1.
Distribution of β-amyloid plaque in the cortex and hippocampus of APP/PS1 mice. Senile plaque formation in 2, 4, 6, 8, 10, and 12-month-old APP/PS1 mouse brain tissues detected by 6E10 immunohistochemistry staining (A). Scale bar in A: 200 mm. Quantification of area occupied by Aβ plaques in the cortex (B) and hippocampus (C) at different ages (n=3 for each group). Error bars represent mean±SEM. *P<0.05, **P<0.01, ***P<0.001 compared with 2-month mice, based on one-way ANOVA followed by Dunnett’s test."
Figure 2.
Distribution of β-amyloid plaque in the cortex and hippocampus of APP/PS1 mice. Senile plaque formation in 2, 4, 6, 8, 10, and 12-month-old APP/PS1 mouse brain tissues assayed by modified Gallyas silver staining (A). Scale bar in A: 200 mm. Quantification of area occupied by Aβ plaques in the cortex (B) and hippocampus (C) at different ages (n = 3 for each group). Error bars represent mean ± SEM. *P<0.05, **P<0.01, ***P<0.001 compared with 2-month mice, based on one-way ANOVA followed by Dunnett’s test."
Figure 3.
Distribution of β-amyloid plaque in the cortex and hippocampus of APP/PS1 mice. Senile plaque formation in 2, 4, 6, 8, 10, and 12-month-old APP/PS1 mouse brain tissue tested by modified thioflavin-S staining (A). Scale bar in A: 200 mm. Quantification of area occupied by Aβ plaques in the cortex (B) and hippocampus (C) at different ages (n=3 for each group). Error bars represent mean ± SEM. *P<0.05, ***P<0.001 compared with two-month mice, based on one-way ANOVA followed by Dunnett’s test."
Figure 4.
Comparative analysis of β-amyloid plaques stained by different methods. Senile plaques in adjacent slices of the same APP/PS1 mouse stained by 6E10 immunohistochemistry (A), modified Gallyas silver staining (B), and modified thioflavin-S staining (C). Quantification of plaque density in the same region (D, n=9 for each group). Error bars represent mean±SEM. *P<0.05, **P<0.01 based on one-way ANOVA followed by Tukey’ s test. n.s.: not significant. Scale bar in A: 200 mm."
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