Chinese Medical Sciences Journal ›› 2012, Vol. 27 ›› Issue (1): 7-10.doi: 10.1016/S1001-9294(12)60015-7

• Original Article • Previous Articles     Next Articles

Spatio-temporal Expression Study of Phosphorylated 70-kDa Ribosomal S6 Kinase (p70S6k) in Mesial Temporal Lobe Epilepsy

Xiao-liang Xing1  , Long-ze Sha1, Yuan Yao2, Yan Shen1, Li-wen Wu2*, and Qi Xu1*   

  1. 1 National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100005, China 2 Department of Neurology, Peking Union Medical College Hospital , Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100730, China
  • Received:2012-02-23 Revised:2012-04-01 Published:2012-03-30 Online:2012-03-30
  • About author:*Corresponding authors Qi Xu Tel: 86-10-65296432, E-mail:; Li-wen Wu Tel: 86-10-69156374, E-mail: wlw1946@
    *Equal contribution to this paper.

ObjectiveTo determine the spatio-temporal expression of p70S6k activation in hippocampus in mesial temporal lobe epilepsy. Methods Temporal lobe epilepsy model was established by stereotaxically unilateral and intrahippocampal injection of kainite acid (KA) in adult male C57BL/6 mice. Latent and chronic epileptogenesis were represented by mice 5 days after KA injection ( n=5) and mice 5 weeks after KA injection ( n=8), respectively. Control mice ( n=5) were injected with saline. Immunohistochemical assays were performed on brain sections of the mice. Results Hippocampus both ipsilateral and contralateral to the KA injection displayed significantly up-regulated pS6 immunoreactivity in dispersed granule cells in 5-day and 5-week model mice. Conclusion The activation of p70S6k is mainly located in the dentate gyrus in KA-induced mouse model of temporal lobe epilepsy, indicating that the activation may be related with the disperse degree and hypertrophy of granule cells.

Key words: mesial temporal lobe epilepsy, p70S6k, kainate acid, immunoreactivity

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