Chinese Medical Sciences Journal ›› 2017, Vol. 32 ›› Issue (2): 83-91.doi: 10.24920/J1001-9294.2017.012
• Original Article • Previous Articles Next Articles
Du Tingting1, †, Liu Na1, †, Gu Bin1, Li Ying1, Yuan Yifang1, Zhang Wei2, Zhang Tong1, *()
Received:
2017-01-22
Published:
2017-06-30
Online:
2017-06-10
Contact:
Zhang Tong
E-mail:kqzhengji301@163.com
Du Tingting, Liu Na, Gu Bin, Li Ying, Yuan Yifang, Zhang Wei, Zhang Tong. Effects of Aging on the Proliferation and Differentiation Capacity of Human Periodontal Ligament Stem Cells△[J].Chinese Medical Sciences Journal, 2017, 32(2): 83-91.
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Table 1
Primer sequences of genes related to osteoblastic differentiation"
Genes name | Sequences of the primers | bp |
---|---|---|
GAPDH | F:5'-TCTGCATCATCCAGGAGCTTATT-3' R:5'-TGATACAGAAGGCAGGTTCACAA-3' | 238 |
ALP | F:5'-AGCTTTCGAAGAACAACGGA-3' R:5'-TCTTGAAATGCTTTGGGTCC-3' | 239 |
Runx-2 | F:5'-CCCGTGGCCTTCAAGGT-3' R:5'-CGTTACCCGCCATGACAGTA-3' | 92 |
Col-1 | F:5'-GCAAGGTGTTGTGCGATGA-3' R:5'-TGGTCGGTGGGTGACTCTG-3' | 235 |
Figure 1.
Cell morphology of periodontal ligament stem cell (PDLSC) in culture (Original magnification:×10).Primary cells of PDLSC in group A (A), group B (B) and group C (C). Cells successfully grew out from PDL tissue. P1 cells of PDLSC (arrows) in group A (D) showed more typical spindle type morphology than those in group B (E) and group C (F)."
Figure 3.
Inverted microscopic graphs of senescence-associated β-galactosidase (SA-β-G) staining (blue) of PDLSC in the three experimental groups. A. group A; B. group B; C. group C. The stained senescent cells in group C excelled in numbers and intensity when compared to group A and B. (Scale: 200 μm)"
Figure 7.
Bar graphs of Real-time RT-PCR measurements for osteogenesis-related genes ALP, col-1 and Runx-2 in the experiment groups. The values are ratios relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and fold changes are expressed as the mean relative quantity (RQ) ± square deviation (SD). *P<0.05."
1. | Zuba-Surma EK, Kucia M, Ratajczak J, et al.“Small stem cells” in adult tissues: very small embryonic-like stem cells stand up. Cytometry A 2009; 75: 4-13. doi:10.1002/ cyto.a.20665. |
2. | Salvi GE,Lawrence HP,Offenbacher S, et al.Influence of risk factors on the pathogenesis of periodontitis. Periodontol 2000 1997; 14: 173-201. doi:10.1111/j.1600-0757. 1997.tb00197.x. |
3. | Chong LY, Chien LY, Chung MC, et al.Controlling the proliferation and differentiation stages to initiate periodontal regeneration. Connect Tissue Res 2013; 54: 101-7. doi: 10.3109/03008207.2012.751985. |
4. | Villar CC,Cochran DL.Regeneration of periodontal tissues: guided tissue regeneration. Dent Clin North Am 2010; 54: 73-92. doi:10.1016/j.cden.2009.08.011. |
5. | Chen FM, Sun HH, Lu H, et al.Stem cell-delivery therapeutics for periodontal tissue regeneration. Biomaterials 2012; 33: 6320-44. doi:10.1016/j.biomaterials.2012. 05.048. |
6. | Seo BM, Miura M, Gronthos S, et al.Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004; 364(9429): 149-55. doi:10.1016 /S0140-6736(04)16627-0. |
7. | Liu N, Shi S, Deng M, et al.High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway. J Bone Miner Res 2011; 26: 2082-95. doi:10.1002/jbmr.440. |
8. | Wheeler HE, Kim SK.Genetics and genomics of human ageing. Philos Trans R Soc Lond B Biol Sci 2011; 366: 43-50. doi:10.1098/rstb.2010.0259. |
9. | Baker DJ, Wijshake T, Tchkonia T, et al.Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders. Nature 2011; 479: 232-6. doi:10.1038/ nature10600. |
10. | Zhang J, An Y, Gao LN, et al.The effect of aging on the pluripotential capacity and regenerative potential of human periodontal ligament stem cells. Biomaterials 2012; 33: 6974-86. doi:10.1016/j.biomaterials.2012.06.032. |
11. | Ryff CD, Heller AS, Schaefer SM, et al.Purposeful Engagement, Healthy Aging, and the Brain.Curr Behav Neurosci Rep 2016; 3: 318-27. doi:10.1007/s40473- 016-0096-z. |
12. | Wang HL, Cooke J.Periodontal regeneration techniques for treatment of periodontal diseases. Dent Clin North Am 2005; 49: 637-59. doi:10.1016/j.cden.2005.03.004. |
13. | Ivanovski S, Gronthos S, Shi S, et al.Stem cells in the periodontal ligament. Oral Dis 2006; 12: 358-63. doi:10.1111/j.1601-0825.2006.01253.x. |
14. | Iwata T, Yamato M, Ishikawa I, et al.Tissue engineering in periodontal tissue. Anat Rec 2014; 297: 16-25. doi:10. 1002/ar.22812. |
15. | Park JY, Jeon SH, Choung PH.Efficacy of periodontal stem cell transplantation in the treatment of advanced periodontitis. Cell Transplant 2011;20: 271-85. doi:10.3727/ 096368910X519292. |
16. | Seo BM, Miura M, Sonoyama W, et al.Recovery of stem cells from cryopreserved periodontal ligament. J Dent Res 2005; 84: 907-12. doi:10.1177/ 154405910508401007. |
17. | Liu Y, Zheng Y, Ding G, et al.Periodontal ligament stem cell-mediated treatment for periodontitis in miniature swine. Stem Cells 2008; 26: 1065-73. doi:10.1634/ stemcells.2007-0734. |
18. | Chen FM, Gao LN, Tian BM, et al.Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: a randomized clinical trial. Stem Cell Res Ther 2016; 7:33. doi:10.1186/s13287-016-0288-1. |
19. | Galderisi U, Helmbold H, Squillaro T, et al.In vitro senescence of rat mesenchymal stem cells is accompanied by downregulation of stemness-related and DNA damage repair genes. Stem Cells Dev 2009; 18: 1033-42. doi:10.1089/scd.2008.0324. |
20. | Røsland GV, Svendsen A, Torsvik A, et al.Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res 2009; 69: 5331-9. doi:10.1158/ 0008-5472.CAN-08-4630. |
21. | Ryu E, Hong S, Kang J, et al.Identification of senescence-associated genes in human bone marrow mesenchymal stem cells. Biochem Biophys Res Commun 2008; 371: 431-6. doi:10.1016/j.bbrc.2008.04.111. |
22. | Ksiazek K.A comprehensive review on mesenchymal stem cell growth and senescence. Rejuvenation Res 2009; 12: 105-16. doi:10.1089/rej.2009.0830. |
23. | Li B, Jung HJ, Kim SM, et al.Human periodontal ligament stem cells repair mental nerve injury. Neural Regen Res 2013; 8: 2827-37. doi:10.3969/ j.issn.1673-5374.2013. 30.005. |
24. | Huang L, Liang J, Geng Y, et al.Directing adult human periodontal ligament-derived stem cells to retinal fate. Invest Ophthalmol Vis Sci 2013; 54: 3965-74. doi:10.1167/ iovs.13-11910. |
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