Chinese Medical Sciences Journal ›› 2017, Vol. 32 ›› Issue (2): 83-91.doi: 10.24920/J1001-9294.2017.012
• Original Article • Previous Articles Next Articles
Effects of Aging on the Proliferation and Differentiation Capacity of Human Periodontal Ligament Stem Cells△
Du Tingting1, †, Liu Na1, †, Gu Bin1, Li Ying1, Yuan Yifang1, Zhang Wei2, Zhang Tong1, *(
)
- 1 Department of Stomatology, Chinese PLA General Hospital, Beijing 100853, China
2 Technical Institute of Physics and Chemistry, Beijing 100190, China
-
Received:2017-01-22Published:2017-06-30Online:2017-06-10 -
Contact:Zhang Tong E-mail:kqzhengji301@163.com
Cite this article
Du Tingting, Liu Na, Gu Bin, Li Ying, Yuan Yifang, Zhang Wei, Zhang Tong. Effects of Aging on the Proliferation and Differentiation Capacity of Human Periodontal Ligament Stem Cells△[J].Chinese Medical Sciences Journal, 2017, 32(2): 83-91.
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Table 1
Primer sequences of genes related to osteoblastic differentiation"
| Genes name | Sequences of the primers | bp |
|---|---|---|
| GAPDH | F:5'-TCTGCATCATCCAGGAGCTTATT-3' R:5'-TGATACAGAAGGCAGGTTCACAA-3' | 238 |
| ALP | F:5'-AGCTTTCGAAGAACAACGGA-3' R:5'-TCTTGAAATGCTTTGGGTCC-3' | 239 |
| Runx-2 | F:5'-CCCGTGGCCTTCAAGGT-3' R:5'-CGTTACCCGCCATGACAGTA-3' | 92 |
| Col-1 | F:5'-GCAAGGTGTTGTGCGATGA-3' R:5'-TGGTCGGTGGGTGACTCTG-3' | 235 |
Table 1
Figure 1.
Cell morphology of periodontal ligament stem cell (PDLSC) in culture (Original magnification:×10).Primary cells of PDLSC in group A (A), group B (B) and group C (C). Cells successfully grew out from PDL tissue. P1 cells of PDLSC (arrows) in group A (D) showed more typical spindle type morphology than those in group B (E) and group C (F)."
Figure 1.
Figure 2.
Gross observations of PDLSC clone formation of the experimental groups. A. group A; B. group B; C. group C. The colony forming efficiency decreased with the increase of donors’ age."
Figure 2.
Figure 3.
Inverted microscopic graphs of senescence-associated β-galactosidase (SA-β-G) staining (blue) of PDLSC in the three experimental groups. A. group A; B. group B; C. group C. The stained senescent cells in group C excelled in numbers and intensity when compared to group A and B. (Scale: 200 μm)"
Figure 3.
Figure 4.
Flow cytometry results of cell cycles of PDLSC in the experimental groups. A. group A; B. group B; C. group C. Percentages of cell in the S + G2/M phase decreased with the increase of donors’ age."
Figure 4.
Figure 5.
Cell apoptosis of PDLSC in the experimental groups.A. group A; B. group B; C. group C. The overall apoptosis rate increased with increasing of donors’ age."
Figure 5.
Figure 6.
Inverted microscopic photographs of alkaline phosphatase (ALP) staining of PDLSC in the experimental groups. (Original magnification:×10). A. group A; B. group B; C. group C. ALP staining decreased with the increase of donors’ age."
Figure 6.
Figure 7.
Bar graphs of Real-time RT-PCR measurements for osteogenesis-related genes ALP, col-1 and Runx-2 in the experiment groups. The values are ratios relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and fold changes are expressed as the mean relative quantity (RQ) ± square deviation (SD). *P<0.05."
Figure 7.
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