Chinese Medical Sciences Journal ›› 2012, Vol. 27 ›› Issue (2): 65-72.

• Original Article •     Next Articles

PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma

Chang-zheng Liu1†*, Wei Liu2†*, Yi Zheng2, Jin-mei Su3, Jing-jing Li2, Lan Yu2, Xiao-dong He2, and Song-sen Chen1*   

  1. 1Department of Biochemistry, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China
    2Department of General Surgery, 3Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China
  • Received:2012-07-05 Revised:2012-07-05 Published:2012-07-05 Online:2012-07-05

Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells.
Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohistochemistry analysis.
Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bile
Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21.

Key words: cholangiocarcinoma, microRNA-21, phosphatase and tensin homolog, programmed cell death 4

Funding:

△Supported by Institute of Basic Medical Sciences (2009PY13 and 2010PYZ18) and the National Natural Science Foundation of China (81100608 and 30901342).

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