Chinese Medical Sciences Journal ›› 2018, Vol. 33 ›› Issue (4): 240-251.doi: 10.24920/003483

• Review • Previous Articles     Next Articles

Current Technologies of Synthetic Biosensors for Disease Detection: Design, Classification and Future Perspectives

Chen Xue, Lv Yi, Wu Rongqian   

  1. National-Local Joint Engineering Research Center for Precision Surgery & Regenerative Medicine, Shaanxi Provincial Center for Regenerative Medicine and Surgical Engineering, Institute of Advanced Surgical Technology and Engineering, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061 China
  • Received:2018-06-07 Accepted:2018-08-17 Published:2018-12-30 Online:2018-10-08
This article systematically reviews the current technologies of simple and complex synthetic biosensors for disease detection, and highlights some synthetic biology strategies that have been developed to target allergy, liver injuries, insulin resistance, Graves' disease, urate homeostasis and inflammation.

Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into them. By overexpressing, blocking and rewiring native gene pathways, synthetic biologists have harnessed this promising technology to reprogram cells to perform diverse tasks such as drug discovery, biopharmaceutical manufacturing, gene therapy and tissue engineering, etc. In this review, we focus on current technologies of synthetic biosensors for disease detection. We start with the design principle of synthetic biosensors. Then we move towards the characteristics of simple synthetic biosensors, which can respond to a single input signal, and complex synthetic biosensors including Boolean gate biosensors, cascade biosensors, time-delay biosensors, oscillator biosensors and hysteretic biosensors, which can respond to more than two input signals and perform complex tasks. Synthetic biosensor has showed great potential in disease detection, but it is still in its infancy stage. More efforts should be made in identifying and constructing clinically relevant regulation systems. Computational tools are also needed in the design process in order to guarantee the precision of the synthetic biosensor. The ultimate goal of a synthetic biosensor is to act as a therapeutic sensor-effector device that connects diagnostic input with therapeutic output and therefore provides all-in-one diagnostic and therapeutic solutions for future gene- and cell-based therapies.

Key words: synthetic biosensor, gene circuit, disease detection

Article information: 本文系统综述了不同类型合成生物传感器的设计原理以及在疾病诊疗方面的最新应用,包括免疫调控、肝损伤、胰岛素抵抗、Grave's病、以及尿酸内稳态等方面。

Funding: Supported by the National Natural Science Foundation of China (81770491) and the Ministry of Education Innovation Team Development Program of China(81770491)

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