Impact of 1, 25-(OH)2D3 on Left Ventricular Hypertrophy in Type 2 Diabetic Rats

Abstract

Objective To investigate the impact of 1, 25-(OH)₂D₃ on left ventricular hypertrophy (LVH) in type 2 diabetic rats.Methods Type 2 diabetic mellitus (DM) model rats were established by intraperitoneally injecting with 30 mg/kg streptozotocin. After 8 weeks, 19 male rats were identified as diabetic with left ventricular hypertrophy (LVH) by ultrasound examination, and randomly assigned into three groups: untreated (DM-LVH, n=7), treated with insulin (DM-LVH+INS, n=6), and treated with 1, 25-(OH)₂D₃(DM-LVH+VD, n=6). Healthy male rats were used as the controls group (n=6). The fasting blood glucose and the insulin level were determined weekly. The left ventricular mass index, myocardial collagen content, collagen volume fraction, and 1, 25-(OH)₂D₃-receptor level were determined by 4 weeks later.Results In the DM-LVH model group, the insulin level was significantly decreased compared with the non-diabetic control group (P<0.05), whereas the blood glucose, left ventricular mass index, myocardial collagen content, collagen volume fraction, and 1, 25-(OH)₂D₃-receptor expression were significantly increased (all P<0.05). In the DM-LVH+INS and DM-LVH+VD groups, the insulin levels were significantly increased compared with the DM-LVH model group (P<0.05), whereas the other parameters were significantly decreased (all P<0.05).Conclusion 1, 25-(OH)₂D₃ could reverse LVH in diabetic rats and that the mechanism may involve stimulating insulin secretion and reducing blood glucose via direct up-regulation of 1, 25-(OH)₂D₃-receptor expression.

Key words: 1, 25-(OH)2D3, left ventricular hypertrophy, type 2 diabetes mellitus, rat

Funding:

△Supported by the Research Fund for Public Health of the Health and Family Planning Commission of Wuhan Municipality (WG13B12)

Ying Fan, Shan-xiao Zhang, Meng Ren, Li-feng Hong, Xiao-ni Yan. Impact of 1, 25-(OH)₂D₃ on Left Ventricular Hypertrophy in Type 2 Diabetic Rats[J].Chinese Medical Sciences Journal, 2015, 30(2): 114-120.

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