Chinese Medical Sciences Journal ›› 2020, Vol. 35 ›› Issue (3): 195-206.doi: 10.24920/003670
• Original Article • Next Articles
Combination of Evodiamine with Berberine Reveals a Regulatory Effect on the Phenotypic Transition of Colon Epithelial Cells Induced by CCD-18Co
Huang Chao1, *(
), Xiang Keming2, Liang Bingjun1, Huang Weixuan1, Zhang Fanjun1, Shao Yuwan1, Wang Xiulian2, Liu Haosheng1, Shen Weizeng1
- 1Department of Traditional Chinese Medicine, Affiliated Bao’an Hospital of Shenzhen, Southern Medical University, Shenzhen 518100, China
2Community Healthy Service Center, Shenzhen Bao’an Traditional Chinese Medicine Hospital Group, Shenzhen 518100, China
-
Received:2019-11-22Published:2020-09-30Online:2020-09-25 -
Contact:Huang Chao E-mail:huangchao06@163.com
| Transdifferentiation exists between stromal cells or between stromal cells and cancer cells. Evodiamine and berberine are predominant pharmacological components of Zuojin pill, a prescription of Traditional Chinese Medicine, playing crucial functions in remolding of tumor microenvironment. In this study, the authors found evodiamine combined with berberine can regulate the differentiation of colon epithelial cells induced by a human colon myofibroblast line CCD-18Co through suppression of activities of TGF-β/Smads signaling. |
Cite this article
Huang Chao, Xiang Keming, Liang Bingjun, Huang Weixuan, Zhang Fanjun, Shao Yuwan, Wang Xiulian, Liu Haosheng, Shen Weizeng. Combination of Evodiamine with Berberine Reveals a Regulatory Effect on the Phenotypic Transition of Colon Epithelial Cells Induced by CCD-18Co[J].Chinese Medical Sciences Journal, 2020, 35(3): 195-206.
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Figure 1.
Chemical structural formula for berberine (A), evodiamine (B), and LY364947 (C)."
Figure 1.
Figure 2.
Morphological observation of HCoEpiCs with phase contrast microscope. The HCoEpiCs were treated by 25% CCD-18Co-derived conditioned medium (CM) for 24 h, and the LY364947 was 5 mg/ml. L-cBerEvo: low concentration-cBerEvo; M-cBerEvo: moderate concentration-cBerEvo; H-cBerEvo: high concentration-cBerEvo."
Figure 2.
Figure 3.
Fluorescent detection of epithelial-mesenchymal transition (EMT) markers including E-cadherin, alpha-smooth muscle actin (α-SMA) and vimentin using a fluorescence microscope (Olympus) equipped with a 960S-Fluor oil immersion lens (1000 x). The cell nuclei was counterstained with 4’, 6-diamidino-2-phenylindole (DAPI) (A). Fluorescence intensity comparison of the above EMT markers (B). The error bar represents the SD (n=3). *P<0.05 versus the control,**P<0.05 versus the CCD-18Co-CM, #P<0.05 versus the CM+L-cBerEvo (ANOVA)."
Figure 3.
Figure 4.
Detection of EMT markers and transcription factors including ZEB1 and Snail using western blotting. The error bar represents the SD (n=3). *P<0.05 versus the control, **P<0.05 versus the CCD-18Co CM, #P<0.05 versus the CM+L-cBerEvo (ANOVA)."
Figure 4.
Figure 5.
Analysis of effects of CM and cBerEvo and LY364947 (LY) on HCoEpiC cells motility by wound-healing assay. A. Area of wound in the HCoEpiC cells is observed by phase contrast microscopy (10×10). B. Analysis of wound area by Image J, and the area of wound is presented by pixels/cm2. The error bar represents the SD (n=3). *P<0.05 versus the control, **P<0.05 versus the CCD-18Co CM, #P<0.05 versus the CM+L-cBerEvo."
Figure 5.
Figure 6.
Detection of main components in Smad signaling by western blotting. A. Protein bands analysis of western blot in the HCoEpiC cells treated by different conditions. B. Analysis of the ratios of p-Smad2/Smad2 and p-Smad3/Smad3 in these groups. The error bar represents the SD (n=3). *P<0.05 versus the Control, **P<0.05 versus the CCD-18Co CM, #P<0.05 versus the CM+L-cBerEvo (ANOVA)."
Figure 6.
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