Figure 1.

SGC-7901 cells were transduced with let-7a lentivi- ral vectors at a multiplicity of infection of 5 (A) and control lentiviral vector (B), respectively. ×200The brightly stained cells are green fluorescent protein positive cells."

Figure 2.

Cell apoptosis of SGC-7901/let-7a (A), SGC-7901/EV cells (B), and SGC-7901 cells (C).The cells were stained with annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI), and the percentages of apoptotic cells (annexin V+ cells) were then assayed using flow cytometric analysis. The lower left quadrant of the flow cytometry histograms indicates the percentage of live cells; the upper left quadrant indicates the percentage of PI positive, necrotic cells; the lower right quadrant shows the percentage of Annexin-V-FITC positive, apoptotic cells; the upper right quadrant reveals the percentage of PI and Annexin positive, late apoptotic cells. The data are the mean of the three different experiments."