Chinese Medical Sciences Journal ›› 2011, Vol. 26 ›› Issue (3): 152-157.doi: 10.1016/S1001-9294(11)60040-0

• Original Article • Previous Articles     Next Articles

Induction of Endothelial Cell Apoptosis by Anti-alpha-enolase Antibody

Hong-bo Yang1, Wen-jie Zheng2*, Xuan Zhang2, Fu-lin Tang2   

  1. 1Department of Endocrinology, 
    2Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China
  • Online:2011-09-24 Published:2011-09-24
  • Contact: Supported by National Natural Science Foundation of China (30400411) E-mail:wenjzheng@ gmail.com

Abstract: Objective To assess the prevalence of anti-alpha-enolase antibody in systemic autoimmune diseases in Chinese patients and its role in endothelial cell apoptosis.
 Methods The reactivity of anti-alpha-enolase antibody in a variety of autoimmune disorders in Chinese patients was evaluated by dot blot assay. Endothelial cell apoptosis was investigated by in vitro incubation of endothelial cells with IgG purified from anti-alpha-enolase antibody-positive sera, with or without pre-incubation with recombinant alpha-enolase. 
Results Anti-alpha-enolase antibody was prevalent in different systemic autoimmune diseases with relatively high reactivity in Chinese patients. In vitro incubation of endothelial cells with IgG containing anti-alpha-enolase antibody induced apoptosis in a time- and dose-dependent manner. Apoptosis was partly inhibited by pre-incubation of the endothelial cells with recombinant alpha-enolase. 
Conclusions Our data suggest that alpha-enolase is a common auto-antigen recognized by anti- endothelial cell antibodies in connective tissue disease. Interaction between alpha-enolase and its autoantibody plays a role in endothelial cell apoptosis. Changes other than cell killing may contribute to the pathogenesis of endothelial damage and microvascular lesions.

Key words: anti-alpha-enolase antibody, apoptosis, endothelial cell

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