合成生物传感器在疾病检测领域的应用技术进展：设计、分类和展望

陈雪,吕毅,吴荣谦

Current Technologies of Synthetic Biosensors for Disease Detection: Design, Classification and Future Perspectives

Chen Xue,Lv Yi,Wu Rongqian

Table 1 Characteristics of various simple synthetic biosensors

Classification of signal	On/Off	Signals	Regulation factor	Regulation factor fused domain	Chimeric regulation factor	Applications	Reference
Antibiotic
	On and Off	Macrolide (erythromycin, clarithromycin, and roxithromycin)	MphR(A)	VP16 and KRAB	ET	Gene therapy, tissue engineering, in vivo gene function analyses, drug discovery, biopharmaceutical manufacturing	5
	On and Off	Pristinamycin I	Pip	VP16 and KRAB	PIT	Compatible with the Tet-OFF system, thus two different gene activities can be controlled in the same cell	6
Amino acid
	On	L-arginine	ArgR	ART	VP16	Gene therapy and manufacturing of protein pharmaceuticals	7
	On	Tryptophan	TrpR	VP16	TRT	Targeted gene expression control	57
Small molecular
	On and Off	Cumate	CymR	VP16	cTA, rcTA	Regulation of gene expression level and duration	58
Gas
	On	Acetaldehyde	AlcR	-	AlcR	Therapeutic transgene dosing and biopharmaceutical manufacturing	59
	On	6-hydroxy-nicotine	HdnoR	VP16	-	Drug-responsive homologs in basic research, therapeutic cell engineering and biopharmaceutical manufacturing.	60
	On	pH/CO2	TDAG8	-	-	Remote control of cellular behavior inside microfluidic devices; CO2-triggered production of biopharmaceuticals in standard bioreactors	61
Drug compound
	On	2-phenylethyl-butyrate	EthR	VP16	-	Generic screening platform to discover drug candidates	62
Qutam sensing signal
	On	Butyrolactones	ScbR SpbR	VP16	SCA, SPA	Clinical application	9
	On	3-oxo-C₈-HSL	TraR	NF-κB p65	p65NTraR	Versatile gene expression control	10
Metabolites
	On	Uric acid	HucR	KRAB	mUTS	Self-sufficient control of pathologic metabolites, gene- and cell-based therapies	54
	On	Bile acids	TGR5	-	p65NTraR	Liver injuries protection	44
	On	Fatty acid	TtgR	-	LSR	Metabolic disorders treatment	63
Food additive
	Off	Phloretin	TtgR	VP16	TtgA	Biopharmaceutical manufacturing, gene- and cell- based therapies	8
	On	Vanillic acid	MOR9-1	-	-	Cell differentiation	64
	On and Off	Vanillic acid	VanR	VP16 and KRAB	VanA₁ and VanA₄	Biopharmaceutical manufacturing, gene- and cell- based therapies	65
Allergic biomarkers
	On	Histamine	HRH2	-	-	Allergy diagnostics	40
	On	Acrylate	AcuR	-	-	Investigate characteristics of inducible transcriptional regulators	66
	On	Glucarate	CdaR	-	-
	On	Erythromycin	MphR	-	-
	On	Naringenin	TtgR	-	-
Vitamin
	Off	Biotin	BirA	-	-	Cell therapy and biopharmaceutical manufacturing	67
	On	Biotin	BirA	VP16	BIT	Gene therapy, tissue engineering, biopharmaceutical manufacturing	68
Hormone	On and Off	Thyroid hormone	TSHR	-	TSR	Thyroid hormone regulation	52
Inflammation factor	On	NF-kB	NF-kB-responsive elements	-	-	Inflammation monitor	56