We report a simple and rapid HLA-DRBI generic typing method

PCRSSP

which is practical and inexpensive. We use 9 sequence-specific primers and 2 group specific Primers to define the HI-ADRB1 specificities DR1

DR2

DR3

DR4

DR5

DR6

DR7

DR8

DR9 and DR10. The HLA DR3

DR5

DR6 and DR8 can be amplified by the two primers of DR3568 and DRB1. The DR6 specificity can be identified by excluding the DR3

DR5 and DR8 when the DR3568 are positive. Any individuals can be typed with some exception: the three pairs of phenotype DR3/DR3 and DR3/DR6

DR5/DRS and DR5/DR6

DR8/DR8 and DR8/DR6 cannot be discriminated from each other. We typed 106 unrelated healthy people from Beijing locations in two weeks. We think this typing method is suitable to replace the error-prone serologic HLA-DR tests in routine clinical practice

including the Prospective typing of cadaveric organ donors.