Influence of Photodynamic Therapy on Apoptosis and Invasion of Human Cholangiocarcinoma QBC939 Cell Line

Chen Yun-jie; Jiang Hai-tao; Cao Jing-yu

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Influence of Photodynamic Therapy on Apoptosis and Invasion of Human Cholangiocarcinoma QBC939 Cell Line

Chinese Medical Sciences Journal Vol. 30, Issue 4, Pages: 252-259(2015)

Orginal Article | Updated：2024-04-10

- Influence of Photodynamic Therapy on Apoptosis and Invasion of Human Cholangiocarcinoma QBC939 Cell Line
- Affiliations：
  
  1. 1Department of General Surgery, Ningbo No. 2 Hospital, Ningbo 315010, Zhejiang, China
  2. 2Department of Hepatobiliary Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong, China
- Author bio：
  
  *Corresponding author E-mail: jht5019@aliyun.com
- Funds：
- DOI：
  CLC：
- Published Online：20 August 2015，
  
  Published：2015-08
- Accepted：
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Chen Yun-jie, Jiang Hai-tao, Cao Jing-yu. Influence of Photodynamic Therapy on Apoptosis and Invasion of Human Cholangiocarcinoma QBC939 Cell Line[J]. Chinese medical sciences journal, 2015, 30(4): 252-259.
DOI：

Chen Yun-jie, Jiang Hai-tao, Cao Jing-yu. Influence of Photodynamic Therapy on Apoptosis and Invasion of Human Cholangiocarcinoma QBC939 Cell Line[J]. Chinese medical sciences journal, 2015, 30(4): 252-259. DOI：

摘要

Abstract

Objective

To investigate the effect of photodynamic therapy (PDT) mediated by hematoporphyrin derivative (HPD) on apoptosis and invasion of cholangiocarcinoma QBC939 cell lines.

Methods

In vitro

cultured cholangiocarcinoma QBC939 cell line was exposed to 2

and 14 μg/ml HPD with 5

and 15 J/cm

light intensity

respectively. The optical density at 450 nm of the QBC939 cells was measured by CCK8 assay and its growth inhibition ratio was calculated. Flow cytometry and transwell migration assay were applied to detect cell apoptosis and invasion respectively. RT-PCR and immunocytochemistry analyses were used to detect expressions of vascular endothelial growth factor-C (VEGF-C)

cyclooxygenase-2 (COX-2)

and proliferating cell nuclear antigen (PCNA). Enzyme-linked immunosorbent assay (ELISA) was carried out to examine the secretion of VEGF-C and COX-2 in QBC939 cells.

Results

Exposure to HPD-PDT can significantly suppress the growth of QBC939 cells (all

0.05). HPD-PDT can promote apoptosis of QBC939 cells at the early stage. When the concentration of HPD was 2 μg/ml and light irradiation was 5 J/cm

HPD-PDT had no obvious inhibitory effect on QBC939 cell growth

but can obviously inhibit cell invasion

and significant difference was observed between the HPD-PDT and control groups (

0.01). The HPD-PDT can reduce the mRNA and protein expressions of VEGF-C

COX-2

and PCNA

and decrease the secretion of VEGF-C and COX-2 in QBC939 cells.

Conclusion

PDT could promote apoptosis and inhibit growth and invasion of cholangiocarcinoma cells QBC939

in vitro

关键词

Keywords

references

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