Up-regulation of Fas Ligand Expression by Sirtuin 1 in both Flow-restricted Vessels and Serum-stimulated Vascular Smooth Muscle Cells<sup>025B3;</sup>

Li Li; Peng Gao; Hou-zao Chen; Zhu-qin Zhang; Ting-ting Xu; Yu-yan Jia; Hui-na Zhang; Guan-hua Du; De-pei Liu

Your Location：

Home >

Browse articles >

Up-regulation of Fas Ligand Expression by Sirtuin 1 in both Flow-restricted Vessels and Serum-stimulated Vascular Smooth Muscle Cells^025B3;

null Issue 2, Pages: 65-71(2013)

Updated：2024-04-10

- Up-regulation of Fas Ligand Expression by Sirtuin 1 in both Flow-restricted Vessels and Serum-stimulated Vascular Smooth Muscle Cells^025B3;
- Affiliations：
  
  1. Beijing Key Laboratory of Drug Target and Screening Research, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100050
  2. ,China
  3. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100005
- Author bio：
- Funds：
- DOI：
  CLC：
- Published Online：26 June 2013，
  
  Published：2013
- Accepted：
Scan QR Code
Li Li, Peng Gao, Hou-zao Chen, et al. Up-regulation of Fas Ligand Expression by Sirtuin 1 in both Flow-restricted Vessels and Serum-stimulated Vascular Smooth Muscle Cells^025B3;[J]. Null, 2013, (2): 65-71.
DOI：

Li Li, Peng Gao, Hou-zao Chen, et al. Up-regulation of Fas Ligand Expression by Sirtuin 1 in both Flow-restricted Vessels and Serum-stimulated Vascular Smooth Muscle Cells^025B3;[J]. Null, 2013, (2): 65-71. DOI：

摘要

SIRT1 and FasL protein levels were detected by Western blotting in either mouse arteries extract or the whole rat aortic vascular smooth muscle cell (VSMC) lysate. Smooth muscle cell (SMC)-specific human SIRT1 transgenic (Tg) C57BL/6 mice and their littermate wild-type (WT) controls underwent complete carotid artery ligation (ligation groups) or the ligation-excluded operation (sham groups). The carotid arteries were collected 1 day after operation. Reverse transcription-polymerase chain reaction was performed to detect the mRNA levels of SIRT1 and FasL. Luciferase reporter assays were performed to detect the effect of WT-SIRT1

a dominant-negative form of SIRT1 (SIRT1H363Y)

and GATA-6 on the promoter activity of FasL. Flow cytometry assay was applied to measure the hypodiploid DNA content of VSMC so as to monitor cellular apoptosis.

Abstract

a dominant-negative form of SIRT1 (SIRT1H363Y)

and GATA-6 on the promoter activity of FasL. Flow cytometry assay was applied to measure the hypodiploid DNA content of VSMC so as to monitor cellular apoptosis.

关键词

Keywords

references

Views

1075

Downloads

CSCD

Alert me when the article has been cited

Submit

Publicity Resources

EXPRESSION OF PERFORIN AND FAS LIGAND IN INFILTRATING CELLS OF MURINE MYOCARDIUM INFECTED WITH COXSACKIEVIRUS B3

INHIBITORY EFFECT OF ANGIOTENSIN II TYPE 1 RECEPTOR-ASSOCIATED PROTEIN ON VASCULAR SMOOTH MUSCLE CELL GROWTH AND NEOINTIMAL FORMATION

OVER-EXPRESSION OF EXTRACELLULAR SIGNAL-REGULATED KINASE IN VASCULAR SMOOTH MUSCLE CELL OF HYPERTENSIVE RATS

Related Author

No data

Related Institution

No data

⁰

Up-regulation of Fas Ligand Expression by Sirtuin 1 in both Flow-restricted Vessels and Serum-stimulated Vascular Smooth Muscle Cells025B3;

DOI：

摘要

Abstract

关键词

Keywords

references

Up-regulation of Fas Ligand Expression by Sirtuin 1 in both Flow-restricted Vessels and Serum-stimulated Vascular Smooth Muscle Cells^025B3;