Mechanical Strain Regulates Osteoblast Proliferation Through Ca<sup>2+</sup>-CaMK-CREB Signal Pathway

Yong Guo; Qi Lv; Xian-qiong Zou; Zhi-xiong Yan; Yu-xian Yan

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Mechanical Strain Regulates Osteoblast Proliferation Through Ca²⁺-CaMK-CREB Signal Pathway

Chinese Medical Sciences Journal Vol. 31, Issue 2, Pages: 100-106(2016)

Updated：2024-04-10

- Mechanical Strain Regulates Osteoblast Proliferation Through Ca²⁺-CaMK-CREB Signal Pathway
- Affiliations：
  
  1. 1Depantment of Bioengineering, College of Biotechnology, Guilin Medical University,Guangxi,Guilin,China,541004
  2. Institute of Medical Equipment, Academy of Military Medical Sciences,Tianjin,China,300161
  3. Experiment Management Center, Logistical College of People Armed Police Forces,Tianjin,China,300162
- Author bio：
- Funds：
- DOI：
  CLC：
- Published Online：20 June 2016，
  
  Published：2016
- Accepted：
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Yong Guo, Qi Lv, Xian-qiong Zou, et al. [J]. Chinese medical sciences journal, 2016, 31(2): 100-106.
DOI：

Yong Guo, Qi Lv, Xian-qiong Zou, et al. [J]. Chinese medical sciences journal, 2016, 31(2): 100-106. DOI：

摘要

Objective To investigate the effects of mechanical strain on Ca-calmodulin dependent kinase (CaMK)-cAMP response element binding protein (CREB) signal pathway and proliferation of osteoblasts.Methods Using a four-point bending device

MC3T3-E1 cells were exposed to mechanical tensile strains of 2500 µs and 5000 µs at 0.5 Hz respectively. The intracellular free Ca ([Ca]i) concentration and calmodulin activity were assayed by fluorospectrophotometry

CaMK II β

CREB

and phosphorylated (activated) CREB (p-CREB) were assessed by Western blot

and cells proliferation was assayed with MTT. Pretreatment with verapamil was carried out to block Ca channel

and inhibitor U73122 was used to inhibit phospholipase C (PLC).Results Mechanical strains of 2500 µs and 5000 µs for 1 to 10 minutes both increased [Ca]i level of the cells. The 2500 µs strain

a periodicity of 1 h/d for 3 days

activated calmodulin

elevated protein levels of CaMK II β and p-CREB

and promoted cells proliferation

which were attenuated by pretreatment of verapamil or U73122. The effects of 5000 µs strain on calmodulin

CaMK II β

p-CREB and proliferation were contrary to 2500 µs strain.Conclusion The mechanical strain regulates osteoblasts proliferation through Ca-CaMK-CREB signal pathwayCa channel and PLC/IP transduction cascades.

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Mechanical Strain Regulates Osteoblast Proliferation Through Ca2+-CaMK-CREB Signal Pathway

DOI：

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Abstract

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references

Mechanical Strain Regulates Osteoblast Proliferation Through Ca²⁺-CaMK-CREB Signal Pathway