PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma

Chang-zheng Liu; Wei Liu; Yi Zheng; Jin-mei Su; Jing-jing Li; Lan Yu; Xiao-dong He; and Song-sen Chen

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PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma

null Vol. 27, Issue 2, Pages: 65-72(2012)

Updated：2024-04-10

- PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma
- Affiliations：
  
  1. Department of Biochemistry, National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100005
  2. Department of General Surgery
  3. Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100730
- Author bio：
- Funds：
- DOI：
  CLC：
- Published Online：05 July 2012，
  
  Published：2012
- Accepted：
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Chang-zheng Liu, Wei Liu, Yi Zheng, et al. [J]. Null, 2012, 27(2): 65-72.
DOI：

Chang-zheng Liu, Wei Liu, Yi Zheng, et al. [J]. Null, 2012, 27(2): 65-72. DOI：

摘要

To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells. The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line

QBC939

was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot

respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR

locked nucleic acid hybridization (LNA-ISH)

and immunohistochemistry analysis. Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bile Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN

PDCD4 are direct effectors of microRNA-21. △Supported by Institute of Basic Medical Sciences (2009PY13 and 2010PYZ18) and the National Natural Science Foundation of China (81100608 and 30901342).

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Related Author

Chang-zheng Liu

Wei Liu

Yi Zheng

Jin-mei Su

Jing-jing Li

Lan Yu

Xiao-dong He

and Song-sen Chen

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Department of General Surgery, Ningbo No. 2 Hospital

Department of Hepatobiliary Surgery, the Affiliated Hospital of Qingdao University

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