Observation of Insulin Exocytosis by a Pancreatic β Cell Line with Total Internal Reflection Fluorescence Microscopy

Zhao-ying Fu; Ya-ping Wang; and Yu Chen

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Observation of Insulin Exocytosis by a Pancreatic β Cell Line with Total Internal Reflection Fluorescence Microscopy

Chinese Medical Sciences Journal Issue 1, Pages: 60-63(2011)

Updated：2024-04-10

- Observation of Insulin Exocytosis by a Pancreatic β Cell Line with Total Internal Reflection Fluorescence Microscopy
- Affiliations：
  
  1College of Medicine, Yanan University , Yanan 716000, China 2 Institute of Biophysics, Chinese Academy of Science,Beijing,China,100101
- Author bio：
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- DOI：
  CLC：
- Published Online：18 April 2011，
  
  Published：2011
- Accepted：
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Zhao-ying Fu, Ya-ping Wang, and Yu Chen. Observation of Insulin Exocytosis by a Pancreatic β Cell Line with Total Internal Reflection Fluorescence Microscopy[J]. Chinese medical sciences journal, 2011, (1): 60-63.
DOI：

Zhao-ying Fu, Ya-ping Wang, and Yu Chen. Observation of Insulin Exocytosis by a Pancreatic β Cell Line with Total Internal Reflection Fluorescence Microscopy[J]. Chinese medical sciences journal, 2011, (1): 60-63. DOI：

摘要

INSULIN secretion was traditionally measured with biochemical and immunological methods such as enzyme linked immunosorbant ?assay and radioimmunoassay. However

these methods can only tell the amount of insulin secreted; they give no information about the secretion process or mechanism of exocytosis. In recent years

an imaging technique known as total internal reflection fluorescence (TIRF) microscopy has been employed to study insulin secretion.1-4 This imaging technique can explore events taking place near or on live cell membrane

such as secretory granule movement

exocytosis

vesicle content release

and membrane fusion.5-10 In the present paper

we applied TIRF microscopy to the observation of insulin exocytosis by the pancreatic β cell line Ins-1.

Abstract

INSULIN secretion was traditionally measured with biochemical and immunological methods such as enzyme linked immunosorbant ?assay and radioimmunoassay. However

these methods can only tell the amount of insulin secreted; they give no information about the secretion process or mechanism of exocytosis. In recent years

such as secretory granule movement

exocytosis

vesicle content release

and membrane fusion.5-10 In the present paper

we applied TIRF microscopy to the observation of insulin exocytosis by the pancreatic β cell line Ins-1.

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