Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation<sup>025B3;</sup>

Rui-feng Yang; Guo-wei Zhao; Shu-ting Liang; Hou-zao Chen; and De-pei Liu

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Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation^025B3;

null Issue 2, Pages: 82-87(2013)

Updated：2024-04-10

- Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation^025B3;
- Affiliations：
  
  National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100005
- Author bio：
- Funds：
- DOI：
  CLC：
- Published Online：26 June 2013，
  
  Published：2013
- Accepted：
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Rui-feng Yang, Guo-wei Zhao, Shu-ting Liang, et al. Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation^025B3;[J]. Null, 2013, (2): 82-87.
DOI：

Rui-feng Yang, Guo-wei Zhao, Shu-ting Liang, et al. Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation^025B3;[J]. Null, 2013, (2): 82-87. DOI：

摘要

Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting were performed to analyze the expression of LSD1 and interleukin-6 (IL-6) in THP-1 monocytes and THP-1-derived macrophages. Chromatin immunoprecipitation (ChIP) assay was applied to detect the occupancy of LSD1 and H3K4 methylation at IL-6 promoter during THP-1 monocyte-to-macrophage differentiation. IL-6 mRNA level and H3K4 methylation at IL-6 promoter were analyzed using qRT-PCR and ChIP assay in LSD1-knockdown THP-1 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) for 0

and 24 hours. Fluorescence activated flow cytometry was performed to reveal the percentage of macrophages differentiated from THP-1 monocytes.

Abstract

and 24 hours. Fluorescence activated flow cytometry was performed to reveal the percentage of macrophages differentiated from THP-1 monocytes.

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Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation025B3;

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Abstract

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Lysine-specific Demethylase 1 Represses THP-1 Monocyte-to-macrophage Differentiation^025B3;