Objective To explore the contrast-enhanced ultrasonographic features for quantitative assessment of hepatic fibrosis. Methods 86 patients with chronic viral hepatitis B were enrolled in this study from March 2007 to August 2009. The patients were classified into 5 groups (S

0

-S

4

) according to fibrosis stage evaluated with ultrasound guided liver biopsy. New contrast-enhanced ultrasonography (CEUS) features including area under the time-intensity curve (TIC) of portal venous phase/hepatic arterial phase (Q

p

/Q

a

) and intensity of portal venous phase/hepatic arterial phase (Ip/Ia) were used to detect the blood supply ratio (portal vein/hepatic artery) in each group. Arrival time of portal vein trunk (T

p

) and decreasing rate of TIC (β) were also analyzed. Results Q

p

/Q

a

and I

p

/I

a

decreased from S

0

to S

4

while T

p

and β increased. These 4 features were significantly correlated with the degree of fibrosis (P

<

0.001) and were significantly different among the five groups (P

<

0.001). Sensitivity and specificity of Ip/Ia were 80% and 86% for groups ≥S1

75% and 86% for groups ≥ S

2

71% and 84% for groups ≥ S

3

and 76% and 80% for group S

4

respectively. Sensitivity and specificity of Q

p

/Q

a

were 70% and 88% for groups ≥ S

1

80% and 76% for groups ≥ S

2

74% and 70% for groups ≥ S

3

and 81% and 95% for group S

4

respectively. Conclusion I

p

/I

a

and Q

p

/Q

a

could be adopted as reliable

non-invasive features for quantitative assessment of hepatic fibrosis.