Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△

Li-shan Lian; Yao-guo Yang; Wei Liu; Li-long Guo; Heng Guan; Chang-wei Liu; Yong-jun Li

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Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△

Chinese Medical Sciences Journal Vol. 27, Issue 3, Pages: 171-175(2012)

Updated：2024-04-10

- Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△
- Affiliations：
  
  Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100730
- Author bio：
- Funds：
- DOI：
  CLC：
- Published Online：08 October 2012，
  
  Published：2012
- Accepted：
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Li-shan Lian, Yao-guo Yang, Wei Liu, et al. Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△[J]. Chinese medical sciences journal, 2012, 27(3): 171-175.
DOI：

Li-shan Lian, Yao-guo Yang, Wei Liu, et al. Zinc Finger Protein-activating Transcription Factor Up-regulates Vascular Endothelial Growth Factor-A Expression in Vitro△[J]. Chinese medical sciences journal, 2012, 27(3): 171-175. DOI：

摘要

To construct the zinc finger protein-activating transcription factor (ZFP-ATF) plasmid and evaluate its efficacy in inducing vascular endothelial growth factor (VEGF) expression in EY.HY926 endothelial cells. Methods Firstly

we constructed the ZFP-ATF plasmid

then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot

finally

we used the RT-PCR to testify whether the ZFP-ATF can stimulate expression of VEGF splice variants. Results The ZFP-ATF DNA sequences were located the multiclone sites of PVAX1 vector between the site of BamHⅠand Xhol.Western blot result showed VEGF expression in EY.HY926 endothelial cells transfected with ZFP-ATF plasmid was significantly higher than that in cells transfected with VEGF165 (19.95±3.95 vs.12.15±1.55 μg/μL

P<0.01).RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165. Conclusion ZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165

which might have beneficial effects in angiogenesis process.

Abstract

we constructed the ZFP-ATF plasmid

then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot

finally

P<0.01).RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165. Conclusion ZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165

which might have beneficial effects in angiogenesis process.

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