Antagomir Dependent MicroRNA-205 Reduction Enhances Adhesion Ability of Human Corneal Epithelial Keratinocytes

Jun Li; Hua Bai; Yong Zhu; Xiao-yan Wang; Fang Wang; Jun-wu Zhang; Robert M. Lavker and Jia Yu

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Antagomir Dependent MicroRNA-205 Reduction Enhances Adhesion Ability of Human Corneal Epithelial Keratinocytes

Chinese Medical Sciences Journal Issue 2, Pages: 65-70(2010)

Updated：2024-04-10

- Antagomir Dependent MicroRNA-205 Reduction Enhances Adhesion Ability of Human Corneal Epithelial Keratinocytes
- Affiliations：
  
  1. Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China,100005
  2. Department of Cardiology, Chongqing First-aid Medical Center,Chongqing,China,400014
  3. Department of Ophthalmology, The Military General Hospital of Beijing PLA,Beijing,China,100700
  4. Department of Dermatology, Feinberg School of Medicine, Northwestern University,Chicago,USA,60611
- Author bio：
- Funds：
- DOI：
  CLC：
- Published Online：10 June 2010，
  
  Published：2010
- Accepted：
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Jun Li, Hua Bai, Yong Zhu, et al. Antagomir Dependent MicroRNA-205 Reduction Enhances Adhesion Ability of Human Corneal Epithelial Keratinocytes[J]. Chinese medical sciences journal, 2010, (2): 65-70.
DOI：

Jun Li, Hua Bai, Yong Zhu, et al. Antagomir Dependent MicroRNA-205 Reduction Enhances Adhesion Ability of Human Corneal Epithelial Keratinocytes[J]. Chinese medical sciences journal, 2010, (2): 65-70. DOI：

摘要

Objective To investigate the effect of microRNA-205 reduction by antagomirs on adhesion ability of normal human corneal epithelial keratinocytes (NHCEKs). Methods Antagomir-205

complementary and inhibitory to microRNA-205

was used to suppress endogenous microRNA-205 in NHCEKs. The adhesion ability of treated NHCEKs was then assessed by cell adhesion assay. Immunoblot and immunohistochemistry were conducted to determine the level of two focal adhesion-related proteins

focal adhesion kinase (FAK) and paxillin (Pax). Phalloidin staining was performed to measure the level of filamentous actin in antagomir-treated NHCEKs. Results Antagomir-205 markedly reduced the level of microRNA-205 in NHCEKs and significantly enhanced adhesion ability of NHCEKs (P<0.01). Further protein analysis validated that inhibition of microRNA-205 increased the number of phosphorylated FAK and phosphorylated Pax

and decreased filamentous actin. Conclusion Our findings suggest that microRNA-205 has down-regulating effect on cell motility in NHCEKs.

Abstract

Objective To investigate the effect of microRNA-205 reduction by antagomirs on adhesion ability of normal human corneal epithelial keratinocytes (NHCEKs). Methods Antagomir-205

complementary and inhibitory to microRNA-205

and decreased filamentous actin. Conclusion Our findings suggest that microRNA-205 has down-regulating effect on cell motility in NHCEKs.

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