Effects of Sunitinib Malate on Growth of Human Bladder Transitional Cell Line T24 <italic style="font-style: italic">In Vitro</italic><sup>△</sup>

Wen Jin; Li Han-zhong; Ji Zhi-gang; Jin Jing

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Effects of Sunitinib Malate on Growth of Human Bladder Transitional Cell Line T24 In Vitro^△

Chinese Medical Sciences Journal Vol. 30, Issue 1, Pages: 51-55(2015)

Orginal Article | Updated：2024-04-10

- Effects of Sunitinib Malate on Growth of Human Bladder Transitional Cell Line T24 In Vitro^△
- Affiliations：
  
  1. 1Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China;
  2. 2Department of Pharmacy, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China;
- Author bio：
  
  **Corresponding author Tel: 86-10-69156073, E-mail: ljjxmc@163.com
- Funds：
- DOI：
  CLC：
- Received：18 June 2014，
  
  Published Online：20 February 2015，
  
  Published：2015-02
- Accepted：
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Wen Jin, Li Han-zhong, Ji Zhi-gang, et al. Effects of Sunitinib Malate on Growth of Human Bladder Transitional Cell Line T24 In Vitro^△[J]. Chinese medical sciences journal, 2015, 30(1): 51-55.
DOI：

Wen Jin, Li Han-zhong, Ji Zhi-gang, et al. Effects of Sunitinib Malate on Growth of Human Bladder Transitional Cell Line T24 In Vitro^△[J]. Chinese medical sciences journal, 2015, 30(1): 51-55. DOI：

摘要

Abstract

Objective

To investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma (TCC)

in vitro

Methods

Human bladder TCC cell line T24 was cultured and exposed to graded concentrations of sunitinib malate for 72 hours

in vitro

to determine the sensitivities to drug. Cell viability was measured by MTT assay. Cell apoptotic morphology was observed by fluorescence microscope following DAPI staining. Band expressions of Fas

Fas ligand

poly (ADP-ribose) polymerase (PARP) and β-actin were analyzed by Western blot. Wound healing process of T24 cells exposed to sunitinib malate was assayed.

Results

Sunitinib malate exerted a concentration-dependent and time-dependent inhibitory effect on the T24 cell lines. Fluorescence microscopy showed that small vacuoles appeared in the nuclei of T24 cells and the vacuoles were bigger with higher drug concentrations. The expressions of Fas ligand and PARP in T24 cells treated with sunitinib malate exhibited a concentration-dependent increase. Moreover sunitinib malate suppressed the wound healing process in a concentration-dependent manner.

Conclusion

Sunitinib malate exerted marked inhibitory activity against bladder cancer cell line T24.

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Keywords

references

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Effects of Sunitinib Malate on Growth of Human Bladder Transitional Cell Line T24 In Vitro△

DOI：

摘要

Abstract

关键词

Keywords

references

Effects of Sunitinib Malate on Growth of Human Bladder Transitional Cell Line T24 In Vitro^△