Total Glycosides of <italic style="font-style: italic">Ranunculus Japonius</italic> Prevent Hypertrophy in Cardiomyocytes <italic style="font-style: italic">via</italic> Alleviating Chronic Ca<sup>2+</sup> Overload

Dai Hong-liang; Jia Gui-zhi; Zhao Song

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Total Glycosides of Ranunculus Japonius Prevent Hypertrophy in Cardiomyocytes via Alleviating Chronic Ca²⁺ Overload

Chinese Medical Sciences Journal Vol. 30, Issue 1, Pages: 37-43(2015)

Orginal Article | Updated：2024-04-10

- Total Glycosides of Ranunculus Japonius Prevent Hypertrophy in Cardiomyocytes via Alleviating Chronic Ca²⁺ Overload
- Affiliations：
  
  1. 1Department of Community Health Nursing, Liaoning Medical University, Jinzhou, Liaoning 121001, China
  2Department of Biochemistry and Molecular Biology, Liaoning Medical University, Jinzhou, Liaoning 121001, China
  3. 3Centre of Scientific Experiment, Liaoning Medical University, Jinzhou, Liaoning 121001, China
- Author bio：
  
  *Corresponding author Tel: 86-416-4605084, E-mail: jy2006hldai@sohu.com, jiaguizhi2006@163.com
- Funds：
- DOI：
  CLC：
- Received：25 July 2014，
  
  Published Online：20 February 2015，
  
  Published：2015-02
- Accepted：
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Dai Hong-liang, Jia Gui-zhi, Zhao Song. Total Glycosides of Ranunculus Japonius Prevent Hypertrophy in Cardiomyocytes via Alleviating Chronic Ca²⁺ Overload[J]. Chinese medical sciences journal, 2015, 30(1): 37-43.
DOI：

Dai Hong-liang, Jia Gui-zhi, Zhao Song. Total Glycosides of Ranunculus Japonius Prevent Hypertrophy in Cardiomyocytes via Alleviating Chronic Ca²⁺ Overload[J]. Chinese medical sciences journal, 2015, 30(1): 37-43. DOI：

摘要

Abstract

Objective

To evaluate the

in vitro

anti-hypertrophic effect of total Glycosides of

Ranunculus Japonius

(TGRJ).

Methods

Neonatal rat cardiomyocytes were cultured and hypertrophy was induced by adminis- trating isoproterenol (ISO

10 µmol/L) or angiotensin Ⅱ (Ang Ⅱ

1 µmol/L) for 48 hours. In the treatment groups

cells were pretreated with TGRJ (0.3 g/L) for 30 minutes prior to hypertrophic stimuli. The anti-hypertrophic effects of TGRJ were examined by measuring cell size

total protein content

and protein synthesis. Intracellular free Ca

concentration ([Ca

]

) was evaluated using fluorescence dye Fura-2/AM. Sacroplasmic/endoplasmic reticulum Ca

ATPase 2a (SERCA2a)

atrial natriuretic peptide (ANP)

B-type natriuretic peptide (BNP)

and beta-myosin heavy chain (β-MHC) protein expression levels were measured by Western blotting . SERCA2a activity was assayed by p-nitrophenal phosphate disodium salt hexahydrate method.

Results

Increased cell size

total protein content

and protein synthesis following ISO or Ang Ⅱ stimulation were significantly inhibited by pretreatment with TGRJ (all

0.05). This anti-hypertrophic effect of TGRJ was confirmed by its suppressing effect on elevated expression of the three hypertrophic related genetic markers

ANP

BNP

and β-MHC. In addition

TGRJ inhibited ISO or Ang Ⅱ induced up-regulation of [Ca

]

under chronic but not acute conditions. And ISO or Ang Ⅱ induced down-regulation of SERCA2a expression and activity was also effectively rectified by TGRJ pretreatment.

Conclusions

The results of present study suggested that TGRJ could prevent ISO or Ang Ⅱ induced cardiac hypertrophy through improving chronic [Ca

]

disorder

might

via

normalizing SERCA2a expression and activity.

关键词

Keywords

references

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Total Glycosides of Ranunculus Japonius Prevent Hypertrophy in Cardiomyocytes via Alleviating Chronic Ca2+ Overload

DOI：

摘要

Abstract

关键词

Keywords

references

Total Glycosides of Ranunculus Japonius Prevent Hypertrophy in Cardiomyocytes via Alleviating Chronic Ca²⁺ Overload