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Propofol can Protect Against the Impairment of Learning-memory Induced by Electroconvulsive Shock
via Tau Protein Hyperphosphorylation in Depressed Rats△- Affiliations：
1. 1Department of Anesthesiology, Fuzhou General Hospital of the People's Liberation Army Nanjing Military Region, Fujian 350025, China
2. 2Department of Anesthesiology, Tianjin Chest Hospital, Tianjin 300222, China
- Author bio：
*Corresponding author Tel: 86-18102022730, E-mail: liuchao74001@126.com
- Funds：
DOI：
CLC：Received：03 January 2015，
Published Online：20 April 2015，
Published：2015-04
- Accepted：
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Liu Wan-fu, Liu Chao. Propofol can Protect Against the Impairment of Learning-memory Induced by Electroconvulsive Shock
via Tau Protein Hyperphosphorylation in Depressed Rats△[J]. Chinese medical sciences journal, 2015, 30(2): 100-107.
摘要
Abstract
Objective
To explore the possible neurophysiologic mechanisms of propofol and N-methyl-D- aspartate (NMDA) receptor antagonist against learning-memory impairment of depressed rats without olfactory bulbs.
Methods
Models of depressed rats without olfactory bulbs were established. For the factorial design in analysis of variance
two intervention factors were included: electroconvulsive shock groups (with and without a course of electroconvulsive shock) and drug intervention groups [intraperotoneal (ip) injection of saline
NMDA receptor antagonist MK-801 and propofol. A total of 60 adult depressed rats without olfactory bulbs were randomly divided into 6 experimental groups (
n
=10 per group): ip injection of 5 ml saline; ip injection of 5 ml of 10 mg/kg MK-801; ip injection of 5 ml of 10 mg/kg MK-801 and a course of electroconvulsive shock; ip injection of 5 ml of 200 mg/kg propofol; ip injection of 5 ml of 200 mg/kg propofol and a course of electroconvulsive shock; and ip injection of 5 ml saline and a course of electroconvulsive shock. The learning-memory abilities of the rats was evaluated by the Morris water maze test. The content of glutamic acid in the hippocampus was detected by high-performance liquid chromatography. The expressions of p-AT8
Ser202
in the hippocampus were determined by Western blot analysis.
Results
Propofol
MK-801 or electroconvulsive shock alone induced learning-memory impairment in depressed rats
as proven by extended evasive latency time and shortened space probe time. Glutamic acid content in the hippocampus of depressed rats was significantly up-regulated by electroconvulsive shock and down-regulated by propofol
but MK-801 had no significant effect on glutamic acid content. Levels of phosphorylated Tau protein p-AT8
Ser202
in the hippocampus was up-regulated by electroconvulsive shock but was reduced by propofol and MK-801 alone. Propofol prevented learning-memory impairment and reduced glutamic acid content and p-AT8
Ser202
levels induced by electroconvulsive shock.
Conclusion
Electroconvulsive shock might reduce learning-memory impairment caused by protein Tau hyperphosphorylation in depressed rats by down-regulating glutamate content.
关键词
Keywords
references
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