Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells <italic style="font-style: italic">In Vitro</italic><sup>△</sup>

Sheng Li; Yang Xu; Ye Ping; Liu Yong-xue; Han Chun-guang

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Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells In Vitro^△

Chinese Medical Sciences Journal Vol. 30, Issue 4, Pages: 245-251(2015)

Orginal Article | Updated：2024-04-10

- Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells In Vitro^△
- Affiliations：
  
  1Department of Geriatric Cardiology, Chinese PLA General Hospital, Beijing 100853, China
  2Department of Pharmacology & Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, China;
- Author bio：
  
  *Corresponding author Tel: 86-10-88626575, Fax: 86-10-88270497, E-mail: yeping301@yahoo.com.cn
- Funds：
- DOI：
  CLC：
- Published Online：20 August 2015，
  
  Published：2015-08
- Accepted：
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Sheng Li, Yang Xu, Ye Ping, et al. Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells In Vitro^△[J]. Chinese medical sciences journal, 2015, 30(4): 245-251.
DOI：

Sheng Li, Yang Xu, Ye Ping, et al. Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells In Vitro^△[J]. Chinese medical sciences journal, 2015, 30(4): 245-251. DOI：

摘要

Abstract

Objective

To explore the effect of atorvastatin on cardiac hypertrophy and to determine the potential mechanism involved.

Methods

in vitro

cardiomyocyte hypertrophy from neonatal rats was induced with angiotensinⅡ (Ang Ⅱ) stimulation. Before AngⅡ stimulation

the cultured rat cardiac myocytes were pretreated with atorvastatin at different concentrations (0.1

and 10 μmol/L). The following parameters were evaluated: the myocyte surface area

H-leucine incorporation into myocytes

mRNA expressions of atrial natriuretic peptide

brain natriuretic peptide

matrix metalloproteinase 9

matrix metalloproteinase 2

and interleukin-1β

mRNA and protein expressions of the δ/β peroxisome proliferator-activated receptor (PPAR) subtypes.

Results

It was shown that atorvastatin could ameliorate Ang Ⅱ-induced neonatal cardiomyocyte hypertrophy in the area of cardiomyocytes

H-leucine incorporation

and the expression of atrial natriuretic peptide and brain natriuretic peptide markedly. Meanwhile

atorvastatin also inhibited the augmented mRNA level of several cytokines in hypertrophic myocytes. Furthermore

the down-regulated expression of PPAR- δ/β at both the mRNA and protein levels in hypertrophic myocytes could be significantly reversed by atorvastatin treatment.

Conclusions

Atorvastatin could improve AngⅡ-induced cardiac hypertrophy and inhibit the expression of cytokines. Such effect might be partly achieved through activation of the PPAR-δ/β pathway.

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references

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Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells In Vitro△

DOI：

摘要

Abstract

关键词

Keywords

references

Effect of Atorvastatin on Expression of Peroxisome Proliferator-activated Receptor Beta/delta in Angiotensin Ⅱ-induced Hypertrophic Myocardial Cells In Vitro^△