Effects of Scutellaria Baicalensis on Activity and Biofilm Formation of Klebsiella Pneumoniae<sup>△</sup>

Chen Wei; Li Bei; Li Shuai; Ou Yi-wen; Ou Qin

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Effects of Scutellaria Baicalensis on Activity and Biofilm Formation of Klebsiella Pneumoniae^△

Chinese Medical Sciences Journal Vol. 31, Issue 3, Pages: 180-184(2016)

Orginal Article | Updated：2024-04-10

- Effects of Scutellaria Baicalensis on Activity and Biofilm Formation of Klebsiella Pneumoniae^△
- Affiliations：
  
  1. 1The First School of Clinical Medicine
  2. 2Department of Medical Microbiology, Hubei University of Medicine, Shiyan City, Hubei 442000, China
- Author bio：
  
  *Corresponding author Tel: 86-719-8891151, E-mail: 151449176@qq.com
- Funds：
- DOI：
  CLC：
- Published Online：20 June 2016，
  
  Published：2016-06
- Accepted：
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Chen Wei, Li Bei, Li Shuai, et al. Effects of Scutellaria Baicalensis on Activity and Biofilm Formation of Klebsiella Pneumoniae^△[J]. Chinese medical sciences journal, 2016, 31(3): 180-184.
DOI：

Chen Wei, Li Bei, Li Shuai, et al. Effects of Scutellaria Baicalensis on Activity and Biofilm Formation of Klebsiella Pneumoniae^△[J]. Chinese medical sciences journal, 2016, 31(3): 180-184. DOI：

摘要

Abstract

Objective

To explore the effects of Scutellaria baicalensis on activity and biofilm formation of Klebsiella pneumonia (Kp).

Methods

The broth and agar dilution methods were carried out to determine minimum inhibitory concentration and minimum bactericidal concentration of Scutellaria baicalensis for TW518. VITEK-32 system was used to assay TW518 susceptibility to antibiotics. Kp biofilms were formed

in vitro

and stained with BacLight Live/Dead stain. The class integron geneⅠ1 mRNA expression was analyzed with RT-PCR.

Results

The minimum inhibitory concentration of Scutellaria baicalensis on TW518 identified as a Kp colony was 32 mg/ml

and minimum bactericidal concentration was 64 mg/ml. Scutellaria baicalensis and broad-spectrum penicillin

cephalosporin

quinolones

or beta-lactamase had synergistic bactericidal effects. Biofilm formation activity of Kp treated with Scutellaria baicalensis was significantly lower than that of the control group. And class integron geneⅠ1 mRNA expression of TW518 was significantly inhibited by Scutellaria baicalensis.

Conclusions

Scutellaria baicalensis has sterilization effect on Kp

and Scutellaria baicalensis could effectively inhibit Kp biofilm formation with prolonged treatment. Scutellaria baicalensis might inhibit Kp biofilm formation through down-regulating integron geneⅠ1 expression.

关键词

Keywords

references

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