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Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis△
- Affiliations：
1. 1Department of Orthopedics,
2. 2 Key Laboratory of Transplant Engineering and Immunology, Regenerative Medicine Research Centre, West China Hospital, Sichuan University, Chengdu 610041, China
- Author bio：
*Corresponding author, Tel: 86-13881878767, E-mail: shenbin_1971@163.com
- Funds：
DOI：
CLC：-
Published Online：20 August 2016，
Published：2016-08
- Accepted：
Scan QR Code
Si Hai-bo, Zeng Yi, Zhou Zong-ke, et al. Expression of miRNA-140 in Chondrocytes and Synovial Fluid of Knee Joints in Patients with Osteoarthritis△[J]. Chinese medical sciences journal, 2016, 31(4): 207-212.
摘要
Abstract
Objective
To investigate the expression of miRNA-140 in chondrocytes and synovial fluid of osteoarthritis (OA) patients
and explore the relationship between the miRNA-140 expression and OA severity.
Methods
This study enrolled 30 OA patients who underwent total knee arthroplasty for chondrocytes sampling and 30 OA patients who underwent intra-articular injection for synovial fluid sampling. All OA patients were grouped into mild [Kellgren and Lawrence (KL) grade 1-2]
moderate (KL grade 3) and severe (KL grade 4)
with 10 in each subgroups for each sampling purposes. 7 non-OA patients and 10 patients with knee injury were collected for cartilage and synovial fluid sampling respectively as control groups. Chondrocytes were isolated from the cartilage tissue and cultured
in vitro
. Quantitative real time PCR for miRNA-140 in chondrocytes and synovial fluid were performed
and the U6 snRNA was used as internal control. The expression difference of miRNA-140 among groups and correlation between the expression and the KL grade of OA were analysed using one-way ANOVA and Spearman test respectively.
Results
The expression of miRNA-140 in chondrocytes of knees in OA patients was reduced than that in normal knees
and the between-group difference was statistically significant (
F
=305.464
P
<
0.001). miRNA-140 could be detected in synovial fluid of both normal knees and OA knees
its relative expression level was reduced in synovial fluid of OA group compared with normal group
and the between-group difference was statistically significant as well (
F
=314.245
P
<
0.001). The relative expression level of miRNA-140 in both chondrocytes and synovial fluid were negatively correlated with the KL grade of OA(
r
=-0.969
P
<
0.001;
r
=-0.970
P
<
0.001).
Conclusion
miRNA-140 could be detected in chondrocytes and synovial fluid of OA patients
and its expression was negatively correlated with the severity of OA.
关键词
Keywords
references
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