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microRNA-18a Promotes Cell Migration and Invasion Through Inhibiting Dicer l Expression in Hepatocellular Carcinoma
In Vitro △- Affiliations：
1.1Institute of Oncology, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China
2.2Department of Oncology, People’s Hospital of Taixing, Taixing, Jiangsu 225400, China
3.3Department of Oncology, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China
4.4Department of Surgerical Oncology, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214062, China
- Author bio：
*Corresponding author Lihua Li Tel: 86-510-88683507, Fax: 86-510-85808820, E-mail: LLHWXSY@aliyun.com; Zijian Guo Tel: 86-510-88682119, E-mail: gzjwxsy@sina.com
- Funds：
DOI：
CLC：-
Published Online：11 April 2017，
Published：2017-04
- Accepted：
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Zhang Xiufen, Yu Bo, Zhang Fuzheng, et al. microRNA-18a Promotes Cell Migration and Invasion Through Inhibiting Dicer l Expression in Hepatocellular Carcinoma
In Vitro △[J]. Chinese medical sciences journal, 2017, 32(1): 34-43.
摘要
Abstract
Objective
To investigate the effects of microRNA-18a (miR-18a) on migration and invasion of hepatocellular carcinoma (HCC) cells
and its possible mechanism associated with Dicer l.
Methods
HepG2 and HepG2.2.15 cells were transfected with miR-18a inhibitor using Lipofectamine. Cell invasion was evaluated by transwell invasion assay
and cell migration was detected by transwell migration and wound-healing assays. Moreover
luciferase reporter assay was used to identify whether Dicer expression was regulated by miR-18a. Real-time RT-PCR and western blot were performed to analyze Dicer 1 expression. In addition
a functional restoration assay was performed to investigate whether miR-18a promotes HCC cell migration and invasion by directly targeting Dicer 1.
Results
miR-18a inhibitor can suppress the migration and invasion of HCC cells. Furthermore
suppression of Dicer l expression by small interfering RNA essentially abolished the inhibition of cell migration and invasion induced by miR-18a inhibitor
restorating these activities to levels similar to the parental HCC cells. Interestingly
suppression of miR-18a in HCC cells resulted in enhanced expression of Dicer l. In addition
the results of a luciferase assay demonstrated targeted regulation of Dicer l by miR-18a.
Conclusion
Our findings suggest that miR-18a promotes migration and invasion of HCC cells by inhibiting Dicer l expression.
关键词
Keywords
references
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