Up-regulation of <italic style="font-style: italic">Let-7a</italic> Expression Induces Gastric Carcinoma Cell Apoptosis <italic style="font-style: italic">In Vitro</italic>

Zhu Yimin; Xu Fuying

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Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro

Chinese Medical Sciences Journal Vol. 32, Issue 1, Pages: 44-47(2017)

Orginal Article | Updated：2024-04-10

- Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro
- Affiliations：
  
  Department of Surgery, College of Medicine and Health, Lishui University/Lishui People’s Hospital, Lishui, Zhejiang 323000, China
- Author bio：
  
  *Corresponding author E-mail: zym330422@163.com
- Funds：
- DOI：
  CLC：
- Published Online：11 April 2017，
  
  Published：2017-04
- Accepted：
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Zhu Yimin, Xu Fuying. Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro[J]. Chinese medical sciences journal, 2017, 32(1): 44-47.
DOI：

Zhu Yimin, Xu Fuying. Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro[J]. Chinese medical sciences journal, 2017, 32(1): 44-47. DOI：

摘要

Abstract

Objective

To investigate effect of

let-7a

gene over-expression on apoptosis of gastric cancer cell lines SGC-7901.

Methods

The stable

let-7a

gene over-expressing SGC-7901 cells

SGC-7901/

let-7a

cells

were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry.

Results

RT-PCR analysis revealed

let-7a

expression in SGC-7901/

let-7a

cells was significantly increased. Cellular apoptosis assay showed that over-expression of

let-7a

could increase apoptosis of SGC-7901 cells (

0.002).

Conclusion

Up-regulating

let-7a

expression promoted apoptosis in SGC-7901 cells.

关键词

Keywords

references

Patterson M , Gaeta X , Loo K , et al. Let-7 miRNAs can act through notch to regulate human gliogenesis. Stem Cell Reports 2014 ; 11 : 758 - 73 .

Papaioannou G , Inloes JB , Nakamura Y , et al. Let-7 and miR-140 microRNAs coordinately regulate skeletal development. Proc Natl Acad Sci USA 2013 ; 110 : 291 - 300 .

Pasquinelli AE. The primary target of let-7 microRNA. Biochem Soc Trans 2013 ; 41 : 821 - 4 .

Kallen AN , Zhou XB , Xu J , et al. The imprinted H19 lncRNA antagonizes let-7 microRNAs. Mol Cell 2013 ; 52 : 101 - 12 .

De Ruyck K , Duprez F , Ferdinande L , et al. A let-7 microRNA polymorphism in the KRAS 3'-UTR is prognostic in oropharyngeal cancer. Cancer Epidemiol 2014 ; 38 : 591 - 8 .

Zhu YM , Zhong ZX , Liu ZM. Significance and relationship between let-7a and gastric mucosa cancerization. World J Gastroenterol 2010 ; 16 : 3325 - 9 .

Zhu YM , Zhong ZX , Liu ZM. Lentiviral vector-mediated upregulation of let-7a inhibits gastric carcinoma cell growth in vitro and in vivo . Scand J Gastroenterol 2011 ; 46 : 53 - 9 .

Zhu YM , Liu ZM , Bai WB. Experimental study on the effect of let-7a in cell cycle of human gastric carcinoma cell line. Chin J Exp Surg 2010 ; 16 : 424 .

Zhu YM , Xiao XY , Dong LR , et al. Differentially expressed proteins identified in overexpressed let-7a gastric carcinoma cells by two-dimensional polyacrylamide gel electrophoresis. Tumor Biol 2012 ; 33 : 1281 - 90 .

Zhang B , Metharom P , Jullie H. The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for prediction gene transfer events. Genet Vaccines Ther 2004 ; 2 : 6 .

Bernard PS , Wittwer CT. Real-time PCR technology for cancer diagnostics. Clin Chem 2002 ; 48 : 1178 - 85 .

Reinhart BJ , Slack FJ , Basson M. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 2000 ; 403 : 901 - 6 .

Abrahante JE , Daul AL , Li M. The Caenorhabditis elegans hunchback-like gene lin-57/hbl-1 controls developmental time and is regulated by microRNAs. Dev Cell 2003 ; 4 : 625 - 37 .

Lin SY , Johnson SM , Abraham M. The C elegans hunchback homolog, hbl-1, controls temporal patterning and is a probable microRNA target. Dev Cell 2003 ; 4 : 639 - 50 .

Slack FJ , Basson M , Liu Z. The lin-41 RBCC gene acts in the C elegans heterochronic pathway between the let-7 regulatory RNA and the LIN-29 transcription factor. Mol Cell 2000 ; 5 : 659 - 69 .

Vella MC , Choi EY , Lin SY. The C elegans microRNA let-7 binds to imperfect let-7 complementary sites from the lin-41 3'UTR. Genes Dev 2004 ; 18 : 132 - 7 .

Yu FY , Yao HR , Zhu PC. Let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell 2007 ; 131 : 1109 - 23 .

Akao Y , Nakagawa Y , Naoe T. let-7 microRNA functions as a potential growth suppressor in human colon cancer cells. Biol Pharm Bull 2006 ; 29 : 903 - 6 .

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Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro

DOI：

摘要

Abstract

关键词

Keywords

references