Phosphoinositide 3-Kinase/Akt and Nuclear Factor-κB Are Involved in Staphylococcus Aureus-induced Apoptosis in U937 Cells

Jia-he Wang1; Yi-jun Zho u2; Li Tian1; and Ping He1 1Department of Geriatrics; Shengjing Hospital; China Medical University; Shenyang 110004; China 2Department of Endocrinology and Metabolism; the Fourth Affiliated Hospital; China Medical University; Shenyang 110032; China

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Phosphoinositide 3-Kinase/Akt and Nuclear Factor-κB Are Involved in Staphylococcus Aureus-induced Apoptosis in U937 Cells

Chinese Medical Sciences Journal Vol. 24, Issue 4, Pages: 231-235(2009)

Updated：2024-04-10

- Phosphoinositide 3-Kinase/Akt and Nuclear Factor-κB Are Involved in Staphylococcus Aureus-induced Apoptosis in U937 Cells
- Affiliations：
- Author bio：
- Funds：
  
  Supported by the Doctor Research Start-up Fund of Liaoning province （20081055）;a grant from the Education Department of Liaoning province （2009A737）
- DOI：
  CLC： R378
- Published：2009
- Accepted：
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[1].Phosphoinositide 3-Kinase/Akt and Nuclear Factor-κB Are Involved in Staphylococcus Aureus-induced Apoptosis in U937 Cells[J].Chinese Medical Sciences Journal,2009,24(04):231-235.

Jia-he Wang1, Yi-jun Zho u2, Li Tian1, et al. Phosphoinositide 3-Kinase/Akt and Nuclear Factor-κB Are Involved in Staphylococcus Aureus-induced Apoptosis in U937 Cells[J]. Chinese medical sciences journal, 2009, 24(4): 231-235.
[1].Phosphoinositide 3-Kinase/Akt and Nuclear Factor-κB Are Involved in Staphylococcus Aureus-induced Apoptosis in U937 Cells[J].Chinese Medical Sciences Journal,2009,24(04):231-235. DOI：

Jia-he Wang1, Yi-jun Zho u2, Li Tian1, et al. Phosphoinositide 3-Kinase/Akt and Nuclear Factor-κB Are Involved in Staphylococcus Aureus-induced Apoptosis in U937 Cells[J]. Chinese medical sciences journal, 2009, 24(4): 231-235. DOI：

摘要

Objective To explore the mechanisms involved in Staphylococcus aureus（S.aureus） invading human monocytic U937 cells.Methods S.aureus were added to U937 cells at multiplicity of infections（MOI） of 20:1 for 0

and 90 minutes

respectively.Cell apoptosis was analyzed with Hoechst 33258 staining and Annexin V-fluorescein isothiocyanate（FITC）/propidium iodide（PI） flow cytometry analysis.Akt and nuclear factor-κB（NF-κB） activities were detected by Western blotting.Results Infection of U937 cells with S.aureus induced rapid cell death in a time-dependent manner

and the cells displayed characteristic features of apoptosis.S.aureus-induced apoptosis was associated with a prominent downregulation of activated（phosphorylated） Akt and NF-κB.The inhibition of phosphorylated Akt by LY294002 led to the inhibition of NF-κB in a dose-dependent manner.Inhibition of Akt with LY294002 caused further increase in apoptosis of U937 cells.Conclusions S.aureus can stimulate the apoptosis of U937 cells.S.aureus induces apoptosis of U937 cells by inhibiting Akt-regulated NF-κB.

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