COMPARISON BETWEEN IMMUNOFLUORESCENCE AND PCR IN DETECTING HUMAN PAPILLOMA VIRUS IN CONDYLOMA ACUMINATA

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COMPARISON BETWEEN IMMUNOFLUORESCENCE AND PCR IN DETECTING HUMAN PAPILLOMA VIRUS IN CONDYLOMA ACUMINATA

Chinese Medical Sciences Journal Vol. 0, Issue 2, Pages: 125-127(2006)

Updated：2024-04-10

- COMPARISON BETWEEN IMMUNOFLUORESCENCE AND PCR IN DETECTING HUMAN PAPILLOMA VIRUS IN CONDYLOMA ACUMINATA
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  CLC： R752.53
- Published：2006
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COMPARISON BETWEEN IMMUNOFLUORESCENCE AND PCR IN DETECTING HUMAN PAPILLOMA VIRUS IN CONDYLOMA ACUMINATA[J]. Chinese medical sciences journal, 2006, 0(2): 125-127.
DOI：

COMPARISON BETWEEN IMMUNOFLUORESCENCE AND PCR IN DETECTING HUMAN PAPILLOMA VIRUS IN CONDYLOMA ACUMINATA[J]. Chinese medical sciences journal, 2006, 0(2): 125-127. DOI：

摘要

Abstract

Objective To compare the effectiveness of immunofluorescence and polymerase chain reaction （PCR） in detecting human papilloma virus （HPV） in condyloma acuminata （CA）. Methods HPVs in CA tissues from 60 patients were detected by immunofluorescence and PCR

respectively. Different subtypes of HPVs were also identified with restriction fragment length polymorphism （RFLP）. Results The positive detective rates of immunofluorescence and PCR were 56.67% （34/60） and 96.67% （58/60）

respectively （P<0.01）. RFLP results showed HPV6 and HPV11 were the main subtypes in the detected virus

which accounted for 98.28%. Conclusion The sensibility of PCR is superior to that of immunofluorescence.

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