Objective To evaluate the significance of several Dermatophagoides pteronyssinus allergen extracts for skin prick test（SPT） in patients allergic to Dermatophagoides pteronyssinus.Methods Two hundred and nineteen patients enrolled in Peking Union Medical College Hospital underwent SPT and serum specific IgE assay to detect the Dermatophagoides pteronyssinus allergen.Three kinds of house dust mite allergen extracts were used for SPT

including the Dermatophagoides pteronyssinus extract prepared by our laboratory（group A）

standardized Dermatophagoides pteronyssinus extract（group B）

and mixed extracts of Dermatophagoides pteronyssinus and Dermatophagoides farinae（group C）.Human serum specific IgE result was regarded as the reference standard for diagnosis of Dermatophagoides pteronyssinus allergy.The receiver operating characteristic（ROC） curve was used to evaluate the diagnostic performance of SPT with the extracts of three groups.Results SPT results showed that the median wheal diameter of group A

group B

and group C was 0.43

0.35

and 0.28 cm

respectively

with significant difference among three groups（P<0.05）.The difference was significant between group A and B（P<0.01） as well as group A and C（P<0.01）

but not between group B and C（P>0.05）.There was no local urticaria or systemic allergic reactions following the procedure of SPT.Local reaction was observed in 5 patients and delayed reaction was in 2 patients of group A.As for group B and C

local reaction occurred in 3 cases and delayed reaction in 2 cases in each group.The area under ROC curve of SPT with extract in group A

group B

and group C was 0.765

0.801

and 0.782

respectively.Based on the detection results of serum specific IgE

the sensitivity of SPT in diagnosis of Dermato-phagoides pteronyssinus allergy with extract of group A

group B

and group C was 92.4%

87.0%

and 81.5%

and the specificity was 60.6%

73.2%

and 74.8%

respectively.Conclusion The Dermatophagoides pteronyssinus extract for SPT prepared by our laboratory offers good sensitivity and specificity comparable to commercially available allergen extracts

and it may be an appropriate candidate for clinical screening and diagnosis of Dermatophagoides pteronyssinus allergy.