COMBINATION OF γ-INTERFERON WITH TRAIL AND CISPLATIN OR ETOPOSIDE INDUCES APOPTOSIS IN HUMAN NEUROBLASTOMA CELL LINE SH-SY5Y

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COMBINATION OF γ-INTERFERON WITH TRAIL AND CISPLATIN OR ETOPOSIDE INDUCES APOPTOSIS IN HUMAN NEUROBLASTOMA CELL LINE SH-SY5Y

Chinese Medical Sciences Journal Vol. 0, Issue 1, Pages: 38-43(2007)

Updated：2024-04-10

- COMBINATION OF γ-INTERFERON WITH TRAIL AND CISPLATIN OR ETOPOSIDE INDUCES APOPTOSIS IN HUMAN NEUROBLASTOMA CELL LINE SH-SY5Y
- Affiliations：
- Author bio：
- Funds：
  
  the National Natural Sciences Foundation of China（39470739）;the Ministry of Public Health Research Foundation（20122167）;the Doctor Startup-Natural Science Foundation of Li-aoning Province （20041047）
- DOI：
  CLC： R96
- Published：2007
- Accepted：
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COMBINATION OF γ-INTERFERON WITH TRAIL AND CISPLATIN OR ETOPOSIDE INDUCES APOPTOSIS IN HUMAN NEUROBLASTOMA CELL LINE SH-SY5Y[J]. Chinese medical sciences journal, 2007, 0(1): 38-43.
DOI：

COMBINATION OF γ-INTERFERON WITH TRAIL AND CISPLATIN OR ETOPOSIDE INDUCES APOPTOSIS IN HUMAN NEUROBLASTOMA CELL LINE SH-SY5Y[J]. Chinese medical sciences journal, 2007, 0(1): 38-43. DOI：

摘要

Abstract

Objective To study the effect of γ-interferon （IFNγ）

tumor necrosis factor related apoptosis inducing ligand （TRAIL）

and cisplatin or etoposide induced apoptosis in human neuroblastoma cell line SH-SY5Y and its possible molecular mechanisms.Methods The expressions of Caspase 8 mRNA and protein were detected with RT-PCR and Western blot analysis. The effects of IFNγ

TRAIL

IFNγ+TRAIL

IFNγ+Caspase 8 inhibitor+TRAIL

IFNγ+cisplatin+TRAIL

and IFNγ+etoposide+TRAIL on the growth and apoptosis of SH-SY5Y cells were detected with the methods of MTT and flow cytometry. The relative Caspase 8 activity was measured with colorimetric assay. Results Caspase 8 was undetectable in SH-SY5Y cells but an increased expression of Caspase 8 mRNA and protein was found after treatment with IFNγ. SH-SY5Y cells themselves were not sensitive to TRAIL

but those expressing Caspase 8 after treatment with IFNγ were. The killing effect of TRAIL on SH-SY5Y cells expressing Caspase 8 was depressed by Caspase 8 inhibitor. Cisplatin and etoposide could enhance the sensitivity of TRAIL on SH-SY5Y cells. The relative Caspase 8 activity of SH-SY5Y cells in IFNγ+TRAIL group was significantly higher than those of control group

IFNγ group

TRAIL group

and inhibitor group （P<0.01）. There was no significant difference among IFNγ+TRAIL group

IFNγ+cisplatin+TRAIL group

and IFNγ+etoposide+TRAIL group.Conclusions IFNγ could sensitize SH-SY5Y cells to TRAIL-induced apoptosis and this may be realized by the upregulation of Caspase 8. Cisplatin and etoposide could enhance the killing effect of TRAIL on SH-SY5Y cells.

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