Objective To explore the effects of SDS

PBS re-dissolvent solutions on fluorescence values of radioallergosorbent test （RAST） inhibition. Methods Dermatophagoides pterronyssinus allergen immunoCAP and UniCAP 100 System were used. The Sera Pool consisted of 20 Dermatophagoides pterronyssinus allergic patients sera

their specific IgE fluorescence values were between 12 505 and 24 776. Results Fluorescence value percentages decreased: 62.9%

54.1%

43.5%

6.7%

3.7%

2.6%

2.2%

and 1.4% respectively

when SDS concentrations were at 2%

1%

0.5%

0.25%

0.1%

0.05%

0.025%

and 0.01%. Fluorescence values decreased more than 5% with SDS concentrations equal to 0.25% or higher. PBS in 0.1 and 0.01 mol/L concentrations decreased fluorescence values 2.9% and 0.9% respectively. Conclusions SDS is a commonly used surfactants in allergen extract and re-dissolvent prepared allergen precipitation for RAST inhibition. Thus effects of surfactants （e.g. SDS） upon the RAST inhibition tests must be considered when they were used as re-dissolvent agents to improve protein resolution in RAST inhibition.