DOWN-REGULATED EXPRESSION OF PTEN GENE AND LOH OF ITS EPIGENETIC MICROSATELLITES IN GASTRIC CARCINOMA

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DOWN-REGULATED EXPRESSION OF PTEN GENE AND LOH OF ITS EPIGENETIC MICROSATELLITES IN GASTRIC CARCINOMA

Chinese Medical Sciences Journal Vol. 0, Issue 4, Pages: 237-242(2003)

Updated：2024-04-10

- DOWN-REGULATED EXPRESSION OF PTEN GENE AND LOH OF ITS EPIGENETIC MICROSATELLITES IN GASTRIC CARCINOMA
- Affiliations：
  
  1. Li Jinyi 1 ,Zheng Huachuan 2 ,Xu Lei 2 ,Yang Xuefei 2 ,Gao Hong 3 and Xin Yan 2, 1 Armed Police General Hospital,Beijing100012 2 The 4th Laboratory,Cancer Institute,The First Affiliated Hospital of China Medical University,Shenyang110001 3 Laboratory of Pediatric Congenital Abnormality,The Second Affiliated Hospital of China Medical University,Shenyang,110004
  2. ,China
  3. The 4th Laboratory
  4. Cancer Institute
  5. The First Affiliated Hospital of China Medical University
  6. Laboratory of Pediatric Congenital Abnormality
  7. The Second Affiliated Hospital of China Medical University
- Author bio：
- Funds：
  
  ThisstudywassupportedbytheNationalNaturalScienceFoundationofChina（No.30070845and30371607）
- DOI：
  CLC： R735.2
- Published：2003
- Accepted：
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DOWN-REGULATED EXPRESSION OF PTEN GENE AND LOH OF ITS EPIGENETIC MICROSATELLITES IN GASTRIC CARCINOMA[J]. Chinese medical sciences journal, 2003, 0(4): 237-242.
DOI：

DOWN-REGULATED EXPRESSION OF PTEN GENE AND LOH OF ITS EPIGENETIC MICROSATELLITES IN GASTRIC CARCINOMA[J]. Chinese medical sciences journal, 2003, 0(4): 237-242. DOI：

摘要

Abstract

Objective.To investigate PTEN expression and loss of heterozygosity（LOH）of its epigenetic microsatel-lites in gastric carcinoma and explore their roles in tumorigenesis and progression of gastric carcinoma.Methods.LOH of epigenetic microsatellites of PTEN（D10S541

D10S583and D10S1687）was exam-ined in advanced gastric carcinomas（n=56）by PCR-SSCP.The mRNA and protein expressions of PTEN gene were evaluated in normal mucosa（n=56）

early（n=11）and advanced carcinomas（n=56）of the stomach using RT?PCR and immunohistochemoistry respectively.PTEN mRNA and protein expressions were compared with clinicopathological staging and lymph node metastasis of tumors.The relationship be-tween PTEN mRNA expression and LOH of microsatellites was discussed

as well as relationship between PTEN mRNA and protein expression.Results.LOH of D10S541

D10S583and D10S1687was found in28.6%（16/56）of advanced gas-tric carcinomas.The positive rates of PTEN expression were80.4%（45/56）

45.5%（5/11）and32.1%（18/56）in normal gastric mucosa

early and advanced gastric carcinomas at mRNA level

while78.6%（44/56）

36.4%（4/11）and28.6%（16/56）at protein level.PTEN mRNA and protein were less fre-quently expressed in early and advanced gastric carcinomas than normal gastric mucosa（P<0.05）.There was negative correlation between PTEN mRNA expression and LOH of microsatellites（P<0.05）.PTEN protein expression paralleled to its mRNA expression（P<0.05）.The PTEN mRNA and protein expres-sions were negatively correlated with lymph node metastasis of advanced gastric carcinomas（P<0.05）.Conclusion.Down?regulated expression of PTEN and frequent LOH of its epigenetic microsatellites might play an important role in gastric carcinogenesis.Reduced PTEN mRNA expression was closely as-sociated with LOH of its epigenetic microsatellites.Altered expression of PTEN might contribute to lymph node metastasis of gastric carcinoma by decreasing cell adhesion and apoptosis

increasing angiogenesis and cell mobility.

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