THE CONSTRUCTION AND EXPRESSION OF RECOMBINANT SHUTTLE PLASMID WITH OM PL1GENE FROM LEPTOSPIRA INTERROGANS SEROVAR LAI STRAIN 017IN BACILLE CALMETTE-GUERIN

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THE CONSTRUCTION AND EXPRESSION OF RECOMBINANT SHUTTLE PLASMID WITH OM PL1GENE FROM LEPTOSPIRA INTERROGANS SEROVAR LAI STRAIN 017IN BACILLE CALMETTE-GUERIN

Chinese Medical Sciences Journal Vol. 0, Issue 2, Pages: 81-84(2002)

Updated：2024-04-10

- THE CONSTRUCTION AND EXPRESSION OF RECOMBINANT SHUTTLE PLASMID WITH OM PL1GENE FROM LEPTOSPIRA INTERROGANS SEROVAR LAI STRAIN 017IN BACILLE CALMETTE-GUERIN
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  CLC： R514
- Published：2002
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THE CONSTRUCTION AND EXPRESSION OF RECOMBINANT SHUTTLE PLASMID WITH OM PL1GENE FROM LEPTOSPIRA INTERROGANS SEROVAR LAI STRAIN 017IN BACILLE CALMETTE-GUERIN[J]. Chinese medical sciences journal, 2002, 0(2): 81-84.
DOI：

THE CONSTRUCTION AND EXPRESSION OF RECOMBINANT SHUTTLE PLASMID WITH OM PL1GENE FROM LEPTOSPIRA INTERROGANS SEROVAR LAI STRAIN 017IN BACILLE CALMETTE-GUERIN[J]. Chinese medical sciences journal, 2002, 0(2): 81-84. DOI：

摘要

Abstract

Objective.To construct recombinant BCG again st leptospirosis.Methods.We amplified the entire open readin g frame of the OmpL1gene from the genome of the leptospire serovar Lai strain 017.Two recombin ant plasmids pBQ1and pBQ2were constructed by oriented ligation based on the E.coli-BCG shuttle plasmids pMV261and pMV361respectively.The recombinant plasmids were transformed into BCG by electroporation.The rBCGs bearing pBQ1and pBQ2were induced by high temperature of 45℃.Results.The expressed product

a 35kD prote in was detected by SDS-PAGE.The resu lt indicates that pBQ1and pBQ2can express OmpL1in rBCG.Conclusion.The technical methods in this study may help detect the immunogenicity a nd immunoprotection of OmpL1and develop more safe

highl y effective rBCG bearing leptospira l antigen with long-lasting protection.

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