Objective To investigate the potential effects of angiogenic process by secretory phospholipase A2 （sPLA2）inhibitor-HyPE（linking N-derivatized phosphatidyl-ethanolamine to hyaluronic acid）on human bone marrow endothelial cell line（HBME-1）. Methods In order to examine the suppressing effects of HyPE on HBME-1 proliferation

migration

and capillary-like tube formation

HBME-1 were activated by angiogenic factor

specifically by basic fibroblast growth factor（b-FGF）

vascular endothelial growth factor（VEGF）

and oncostatin M（OSM）（at a final concentration of 25

20

and 2.5 ng/mL

respectively）

then HBME-1 proliferation

migration

and tube forma-tion were studied in the absence or presence of HyPE. HBME-1 tube formation was specially analyzed in fibrin gel. Results HyPE effectively inhibited HBME-1 proliferation and migration as a dose-dependent manner

whatever HBME-1 were grown in the control culture medium or stimulated with b-FGF

VEGF

or OSM. In fibrin

the formations of HBME-1 derived tube-like structures were enhanced by all angiogenic factors

but these were strongly suppressed by HyPE. Conclusions The results support the involvement of sPLA2 in angiogenesis. It is proposed that sPLA2 inhibitor introduces a novel approach in the control of cancer development.