Objective To examine the clinical application of pulsed Doppler tissue imaging（DTI）for regional left ventricular function assessment in normal subjects. Methods We examined 50 healthy subjects（range 12-42 years

mean age 28.3 ± 6.9 years）using pulsed Doppler tissue imaging to characterize the diastolic and systolic velocity profiles of mitral annulus. Recordings were made along the long axis in the apical 4-chamber

2-chamber

and long apical views of 6 sites（posterior-septum

lateral

anterior

inferior

anterior-septum

posterior）at the mitral annulus. Myocardial velocities were determined with use of variance F statistical analysis. Correlation analysis was employed to test the relationship between age and mitral annular velocities. Results Both early diastolic and systolic velocities at the septum were lower than other sites. There were no differences in mitral annulus late diastolic velocities. Mean early diastolic and systolic velocities was negatively correlated with age. Conclusions Doppler tissue imaging can directly reflect regional left ventricular function.