To investigate the effects of coxsackievirus B 3（CVB 3） on ion channel currents in rat ventricular myocytes. Methods.Rat hearts were isolated with collagenase to acquire single ventricular myocytes

L type voltage dependent calcium channel（VDCC）current （I Ca ）

Na + current （I Na ）

outward potassium current （I out ）

inwardly rectifying potassium current（I KI ） were recorded using whole cell patch clamp techniques. ResultsCVB 3 infection increased I Ca and I out

while decreased I KI ; but it had no obvious effect on I Na . Conclusion.The effects of CVB 3 on I Ca 、 I out 、 I KI may be one of the mechanisms of myocytes damage and the occurrence of abnormal electroactivities induced by CVB 3 infection.